Cloned (Comment) | Organism |
---|---|
gene pac, overexpression in Escherichia coli strain HB101 and secretion of the enzyme to the culture medium, method optimization, overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | Escherichia coli | - |
phenylacetate + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | Escherichia coli JE5505 | - |
phenylacetate + 6-aminopenicillanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli JE5505 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme 3.2fold from Escherichia coli strain HB101 culture supernatant by anion exchange chromatography, single-step purification | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.3 | - |
purified recombinant enzyme, pH 8.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | - |
Escherichia coli | phenylacetate + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | - |
Escherichia coli JE5505 | phenylacetate + 6-aminopenicillanate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAC | - |
Escherichia coli |
penicillin G acylase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |