Any feedback?
Literature summary for 3.5.1.11 extracted from
- Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library (2014), Biotechnol. Lett., 36, 617-625.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PGA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, cloning and recombinant expression of His-tagged enzyme in Escherichia coli strain M15 | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits 15.1% at 1 mM | uncultured bacterium |  |
| Cu2+ | inhibits 61.7% at 1 mM | uncultured bacterium | |
| dimethylformamide | inhibits 10.5% at 10% and 33.2% at 40% | uncultured bacterium | |
| EDTA | inhibits 80.4% at 10 mM | uncultured bacterium | |
| Fe3+ | inhibits 96.0% at 1 mM | uncultured bacterium | |
| Mn2+ | inhibits 66.2% at 1 mM | uncultured bacterium | |
| SDS | inhibits 56.0% at 2% | uncultured bacterium | |
| Sr2+ | inhibits 95.4% at 1 mM | uncultured bacterium | |
| Urea | inhibits 95.7% at 8 M, no inhibition at 4 M | uncultured bacterium | |
| Zn2+ | inhibits 12.5% at 1 mM | uncultured bacterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
penicillin G | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.0083 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.0156 | - |
cephalothin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.0164 | - |
cephaloridine | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.0255 | - |
cephalexin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.0393 | - |
Penicillin V | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.0519 | - |
carbenicillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.0944 | - |
ampicillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 0.105 | - |
amoxycillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates 87.8% at 1 mM | uncultured bacterium | |
| Mg2+ | activates 46.0% at 1 mM | uncultured bacterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
| 63000 | - |
1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
| 88000 | - |
recombinant His-tagged enzyme, gel filtration | uncultured bacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | uncultured bacterium | - |
phenylacetate + 6-aminopenicillanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | D6R1Q1 | PGA gene from a metagenomic library of an Antarctic sample | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain M15 by affinity chromatography and gel filtration | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
uncultured bacterium | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| amoxycillin + H2O | - |
uncultured bacterium | D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid | - |
? | |
| ampicillin + H2O | - |
uncultured bacterium | 6-aminopenicillanic acid + (R)-2-phenylglycine | - |
? | |
| carbenicillin + H2O | - |
uncultured bacterium | phenylpropanedioate + 6-aminopenicillate | - |
? | |
| cephalexin + H2O | - |
uncultured bacterium | 7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + D-phenylglycine | - |
? | |
| cephaloridine + H2O | - |
uncultured bacterium | thiophen-2-ylacetic acid + (6R,7R)-7-amino-8-oxo-3-(pyridinium-1-yl-methyl)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
| cephalothin + H2O | - |
uncultured bacterium | thiophen-2-ylacetic acid + (6R,7R)-3-acetoxymethyl-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
| penicillin G + H2O | - |
uncultured bacterium | phenylacetate + 6-aminopenicillanate | - |
? | |
| penicillin V + H2O | - |
uncultured bacterium | phenoxyacetate + 6-aminopenicillinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACPGA001 PGA | - |
uncultured bacterium |
| penicillin G acylase | - |
uncultured bacterium |
| PGA | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant His-tagged enzyme, at pH 8.0 | uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | activity range | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 90% activity remaining | uncultured bacterium |
| 50 | - |
purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 50% activity remaining | uncultured bacterium |
| 55 | - |
purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 40% activity remaining, t1/2 is 60 min | uncultured bacterium |
| 60 | - |
purified recombinant His-tagged enzyme, pH 7.4, 15 min, inactivation | uncultured bacterium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34.2 | - |
cephalexin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 48.4 | - |
Penicillin V | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 49.5 | - |
ampicillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 56.2 | - |
amoxycillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 58.3 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 63.7 | - |
carbenicillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 64.2 | - |
penicillin G | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 66.9 | - |
cephaloridine | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 78.3 | - |
cephalothin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant His-tagged enzyme, at 60°C | uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range | uncultured bacterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | purified recombinant His-tagged enzyme, 30°C, 30 min, over 80% activity within this range | uncultured bacterium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 524.4 | - |
ampicillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 534.2 | - |
amoxycillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 1227 | - |
carbenicillin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 1232 | - |
Penicillin V | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 1341 | - |
cephalexin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 4079 | - |
cephaloridine | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 5019 | - |
cephalothin | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 7024 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
| 13960 | - |
penicillin G | pH 7.4, 37°C, recombinant enzyme | uncultured bacterium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
