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Literature summary for 3.5.1.11 extracted from

  • Zhang, D.; Koreishi, M.; Imanaka, H.; Imamura, K.; Nakanishi, K.
    Cloning and characterization of penicillin V acylase from Streptomyces mobaraensis (2007), J. Biotechnol., 128, 788-800.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Streptomyces lividans Streptomyces mobaraensis

Inhibitors

Inhibitors Comment Organism Structure
6-aminopenicillanic acid
-
Streptomyces mobaraensis
phenoxyacetic acid
-
Streptomyces mobaraensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
capsaicin
-
Streptomyces mobaraensis
1.6
-
2-nitro-5-phenoxyacetamide benzoic acid
-
Streptomyces mobaraensis
5.2
-
Penicillin V
-
Streptomyces mobaraensis
12
-
penicillin G
-
Streptomyces mobaraensis

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis Q0R3W5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces mobaraensis

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Streptomyces mobaraensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitro-5-phenoxyacetamide benzoic acid + H2O
-
Streptomyces mobaraensis ?
-
?
capsaicin + H2O
-
Streptomyces mobaraensis ?
-
?
penicillin G + H2O
-
Streptomyces mobaraensis 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin V + H2O
-
Streptomyces mobaraensis 6-aminopenicillanic acid + phenoxyacetic acid
-
?

Subunits

Subunits Comment Organism
dimer
-
Streptomyces mobaraensis

Synonyms

Synonyms Comment Organism
penicillin V acylase
-
Streptomyces mobaraensis
Sm-PVA
-
Streptomyces mobaraensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Streptomyces mobaraensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.5
-
capsaicin
-
Streptomyces mobaraensis
12
-
penicillin G
-
Streptomyces mobaraensis
106.4
-
2-nitro-5-phenoxyacetamide benzoic acid
-
Streptomyces mobaraensis
270.8
-
Penicillin V
-
Streptomyces mobaraensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
substrate: beta-lactam antibiotics Streptomyces mobaraensis
7.5
-
substrate: 2-nitro-5-phenoxyacetamide benzoic acid or capsaicin Streptomyces mobaraensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
6-aminopenicillanic acid
-
Streptomyces mobaraensis
11
-
phenoxyacetic acid
-
Streptomyces mobaraensis