Literature summary for 3.5.1.11 extracted from

Pinotti, L.M.; Ribeiro de Souza, V.; de Campos Giordano, R.; de Lima Camargo Giordano, R.
The penicillin G acylase production by B. megaterium is amino acid consumption dependent (2007), Biotechnol. Bioeng., 97, 346-353.

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Organism

Organism	UniProt	Comment	Textmining
Priestia megaterium	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition	51 g/l of casein hydrolyzed with Alcalase and 2.7 g/l of phenylacetic acid (PhAc), the following carbon substrates are tested, individually and combined: glucose, glycerol, and lactose (present in cheese whey). Glycerol and glucose are effective nutrients for the microorganism growth but delay the penicillin G acylase production. Cheese whey always increases enzyme production and cell mass. However lactose (present in cheese whey) is not a significant carbon source for Bacillus megaterium. Phenylacetic acid, amino acids, and small peptides present in the hydrolyzed casein are the actual carbon sources for enzyme production. Replacement of hydrolyzed casein by free amino acids, 10.0 g/l, leads to a significant increase in enzyme production (approximately 150%), with a preferential consumption of alanine, aspartic acid, glycine, serine, arginine, threonine, lysine, and glutamic acid. A decrease of the enzyme production is observed when 20.0 g/l of amino acids are used. Using the single omission technique, it is shown that none of the 18 tested amino acids is essential for enzyme production. The use of a medium containing eight of the preferentially consumed amino acids leads to similar enzyme production level obtained when using 18 amino acids. Phenylacetic acid, up to 2.7 g/l, does not inhibit enzyme production, even if added at the beginning of the cultivation	Priestia megaterium	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
penicillin-G + H2O	-	Priestia megaterium	6-aminopenicillanic acid + phenylacetic acid	-	?

Synonyms

Synonyms	Comment	Organism
penicillin G acylase	-	Priestia megaterium

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Release 2023.1 (January 2023)