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Literature summary for 3.5.1.11 extracted from
- Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis (2007), Appl. Environ. Microbiol., 73, 5378-5381.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | AuAAC should be considered an industrial biocatalyst with high potential in the production of semisynthetic penicillins | Actinoplanes utahensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Streptomyces lividans | Actinoplanes utahensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
penicillin K | pH 8.0, 45°C | Actinoplanes utahensis |  |
| 5.6 | - |
penicillin dihydroF | pH 8.0, 45°C | Actinoplanes utahensis | |
| 15.1 | - |
penicillin F | pH 8.0, 45°C | Actinoplanes utahensis | |
| 15.4 | - |
Penicillin V | pH 8.0, 45°C | Actinoplanes utahensis | |
| 155.8 | - |
penicillin G | pH 8.0, 45°C | Actinoplanes utahensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinoplanes utahensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Actinoplanes utahensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42.8 | - |
- |
Actinoplanes utahensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin dihydroF + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + hexanoic acid | - |
? | |
| penicillin F + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + (3E)-hex-3-enoic acid | - |
? | |
| penicillin G + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| penicillin K + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + octanoic acid | - |
? | |
| penicillin V + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AuAAC | - |
Actinoplanes utahensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Actinoplanes utahensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
20 min, stable up to | Actinoplanes utahensis |
| 82 | - |
aculeacin A acylase is highly thermostable, with a midpoint transition temperature of 81.5°C | Actinoplanes utahensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
penicillin F | pH 8.0, 45°C | Actinoplanes utahensis | |
| 2.2 | - |
penicillin G | pH 8.0, 45°C | Actinoplanes utahensis | |
| 4.2 | - |
penicillin dihydroF | pH 8.0, 45°C | Actinoplanes utahensis | |
| 33.3 | - |
penicillin K | pH 8.0, 45°C | Actinoplanes utahensis | |
| 70.3 | - |
Penicillin V | pH 8.0, 45°C | Actinoplanes utahensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | substrate: penicillin V | Actinoplanes utahensis |
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