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Literature summary for 3.5.1.11 extracted from

  • Basso, A.; Braiuca, P.; Cantone, S.; Ebert, C.; Linda, P.; Spizzo, P.; Caimi, P.; Hanefeld, U.; Degrassi, G.; Gardossi, L.
    In silico analysis of enzyme surface and glycosylation effect as a tool for efficient covalent immobilisation of CalB and PGA on Sepabeads (2007), Adv. Synth. Catal., 349, 877-886.
No PubMed abstract available
Search for more literature for 3.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Providencia rettgeri

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-
Providencia rettgeri
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylpenicillin + H2O
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 Escherichia coli phenylacetic acid + 6-aminopenicillanate
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 ?
benzylpenicillin + H2O higher activity of enzyme immobilised on the amino support suggests that interactions occurring between the amino carrier and the glycan favours a more correct orientation of the protein making it accessible to substrates Providencia rettgeri phenylacetic acid + 6-aminopenicillanate
-
 ?

Synonyms

Synonyms Comment Organism
penicillin G acylase
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 Escherichia coli
penicillin G acylase
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 Providencia rettgeri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 enzyme immobilized on epoxy polymer: 80% loss of activity after 30 min. Enzyme immobilized on amino polymers with short spacer arms: about 80% loss of activity after 60 min. Enzyme immobilized on amino polymers with long spacer arm: about 95% loss of activity after 5 min, about 80% loss of activity after 120 min. Native enzyme in solution: complete loss of activity after 5 min Escherichia coli
60
-
 enzyme immobilized on epoxy polymer: 90% loss of activity after 10 min. Enzyme immobilized on amino polymers with short spacer arms: about 65% loss of activity after 60 min, about 80% loss of activity after 180 min. Enzyme immobilized on amino polymers with long spacer arm: about 60% loss of activity after 60 min, about 80% loss of activity after 120 min. Native enzyme in solution: about 50% loss of activity after 45 min, about 90% loss of activity after 75 min Providencia rettgeri