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Literature summary for 3.5.1.11 extracted from
- One-step purification of poly-His tagged penicillin G acylase expressed in E. coli (2004), J. Microbiol. Biotechnol., 14, 231-236.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | purification of His-tagged enzyme secreted into periplasmic space by immobilized metal-ion affnity chromatography. Maximum specific activity of enzyme is 38.5 U/mg, yield is 70% | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of His-tagged enzyme secreted into periplasmic psace by immobilized metal-ion affnity chromatography. Maximum specific activity of enzyme is 38.5 U/mg, yield is 70% | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38.5 | - |
40°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanic acid + phenylacetic acid | - |
? |  |
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