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Literature summary for 3.5.1.11 extracted from

  • Guranda, D.T.; Volovik, T.S.; Svedas, V.K.
    pH Stability of penicillin acylase from Escherichia coli (2004), Biochemistry (Moscow), 69, 1386-1390.
    View publication on PubMed
Search for more literature for 3.5.1.11

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
-
 Escherichia coli 3-amino-6-nitrobenzoic acid + phenylacetic acid
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 study on inactivation kinetics over a wide pH-range at 25°C and 50°C. At temperatures above 35°C, enzyme undergoes a conformational change accompanied by destruction of salt bridges that maintain the active conformation Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 6-nitro-3-[(phenylacetyl)amino]benzoic acid pH 10.0, 25°C, ionic strength 0.12 M Escherichia coli
26
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 6-nitro-3-[(phenylacetyl)amino]benzoic acid pH 9.1, 25°C, ionic strength 0.12 M Escherichia coli
27
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 6-nitro-3-[(phenylacetyl)amino]benzoic acid pH 8.0, 25°C, ionic strength 0.12 M Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 study on inactivation kinetics over a wide pH-range at 25°C and 50°C. Enzyme is very stable in neutral solutions and quickly loses its catalytic activity in acidic and alkaline solutions. Inactivation proceeds according to first order reaction kinetics Escherichia coli
8
-
 half-life at 25°C is 6 days Escherichia coli
9.1
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 half-life at 25°C is 9.1 h Escherichia coli
10
-
 half-life at 25°C is 0.9 h Escherichia coli