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Literature summary for 3.5.1.11 extracted from

  • Oh, B.; Kim, K.; Park, J.; Yoon, J.; Han, D.; Kim, Y.
    Modifying the substrate specificity of penicillin G acylase to cephalosporin acylase by mutating active-site residues (2004), Biochem. Biophys. Res. Commun., 319, 486-492.
    View publication on PubMed
Search for more literature for 3.5.1.11

Protein Variants

Protein Variants Comment Organism
V56R mutation of beta-subunit, 12% of wild-type activity with substrate penicillin G, 230% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid Escherichia coli
V56R mutation of beta-subunit, plus F146Y in alpha-subunit, 5% of wild-type activity with substrate penicillin G, 280% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid Escherichia coli
V56R/T32Y mutation of beta-subunit, plus F146Y in alpha-subunit, 2.3% of wild-type activity with substrate penicillin G, 460% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid Escherichia coli
V56R/T32Y/I177Y mutation of beta-subunit, plus F146Y in alpha-subunit, 1.8% of wild-type activity with substrate penicillin G, 490% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid Escherichia coli
V56R/T32Y/I177Y/P49Q mutation of beta-subunit, plus F146Y in alpha-subunit, 1.3% of wild-type activity with substrate penicillin G, 510% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid Escherichia coli
V56R/T32Y/I177Y/P49Q/W154Y mutation of beta-subunit, plus F146Y in alpha-subunit, 1.2% of wild-type activity with substrate penicillin G, 600% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid Escherichia coli
V56R/T32Y/I177Y/P49Q/W154Y/F24L mutation of beta-subunit, plus F146Y in alpha-subunit, 0.3% of wild-type activity with substrate penicillin G, 760% of wild-type activity with cephalosporin acylase substrate glutaryl-7-aminocephalosporanic acid Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
 substrate glutaryl-7-aminocephalosporanic acid, wild-type, pH 8.0 Escherichia coli
666
-
 substrate penicillin G, wild-type, pH 8.0 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
 Escherichia coli glutarate + 7-aminocephalosporanic acid
-
 ?
penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanic acid + phenylacetic acid
-
 ?