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Literature summary for 3.5.1.11 extracted from
- Penicillin acylase (bacterial) (1975), Methods Enzymol., 43, 705-721.
General Stability
| General Stability | Organism |
|---|---|
| immobilized enzyme, higher stability than soluble enzyme | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-aminopenicillanic acid | non-competitive inhibition | Escherichia coli |  |
| 6-aminopenicillanic acid | non-competitive inhibition | Priestia megaterium | |
| benzyl penicillin | - |
Escherichia coli | |
| phenylacetic acid | competitive inhibition | Escherichia coli | |
| phenylacetic acid | competitive inhibition | Priestia megaterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
benzylpenicillin | - |
Escherichia coli | |
| 4.5 | - |
benzylpenicillin | - |
Priestia megaterium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Priestia megaterium | - |
- |
| intracellular | - |
Escherichia coli | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Escherichia coli |
| 71000 | - |
sedimentation equilibrium | Escherichia coli |
| 120000 | - |
sedimentation equilibrium | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Priestia megaterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Priestia megaterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 48 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, freeze dried pure enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-furylmethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + furan-2-yl-acetic acid | - |
? | |
| 2-thienylmethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + sulfo-(5-sulfothiophen-2-yl)-acetic acid | - |
? | |
| benzylpenicilloic acid + H2O | - |
Escherichia coli | ? | - |
? | |
| D-alpha-aminobenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + DL-alpha-aminophenylacetic acid | - |
? | |
| DL-alpha-hydroxybenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + DL-alpha-hydroxyphenylacetic acid | - |
? | |
| isobutoxymethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + carbonic acid isopropyl ester | - |
? | |
| additional information | overview on best substrates for similar organisms | Escherichia coli | ? | - |
? | |
| additional information | overview on best substrates for similar organisms | Priestia megaterium | ? | - |
? | |
| N-phenylacetyl-DL-leucine + H2O | - |
Escherichia coli | ? | - |
? | |
| N-phenylacetyl-L-alpha-aminophenylacetic acid + H2O | - |
Escherichia coli | ? | - |
? | |
| N-phenylacetylglycine + H2O | - |
Escherichia coli | ? | - |
? | |
| n-propoxymethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + carbonic acid monopropyl ester | - |
? | |
| p-hydroxybenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + p-hydroxyphenylacetic acid | - |
? | |
| penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
r | |
| penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanate + phenylacetic acid | - |
? | |
| phenylacetamide + H2O | - |
Escherichia coli | ? | - |
? | |
| phenylacetamide + H2O | - |
Priestia megaterium | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview on values for similar organisms | Escherichia coli |
| additional information | - |
overview on values for similar organisms | Priestia megaterium |
| 37 | - |
generally chosen because enzyme unstable at 45°C and 55°C | Escherichia coli |
| 37 | - |
generally chosen because enzyme unstable at 45°C and 55°C | Priestia megaterium |
| 45 | - |
benzylpenicillin | Priestia megaterium |
| 55 | - |
benzylpenicillin | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview on values for similar organisms | Escherichia coli |
| additional information | - |
overview on values for similar organisms | Priestia megaterium |
| 8.2 | - |
- |
Escherichia coli |
| 8.5 | - |
- |
Priestia megaterium |
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