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Literature summary for 3.5.1.108 extracted from

  • Miller, M.; Gao, N.; Ross, P.; Olivier, N.
    Crystal structure of A. aeolicus LpxC with bound product suggests alternate deacetylation mechanism (2015), Proteins, 83, 1706-1719 .
    View publication on PubMed
Search for more literature for 3.5.1.108

Application

Application Comment Organism
medicine as it is essential for the survival of many pathogens, the enzyme is an attractive target for antibacterial therapeutics Aquifex aeolicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, co-crystal structure of the enzyme is solved to 1.6 A resolution Aquifex aeolicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a mechanism is suggested that includes a transition state pentacoordinate system while the zinc is tetracoordinated in the absence of substrate Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-alpha-D-glucosamine + H2O Aquifex aeolicus the enzyme catalyzes the first committed step in the biosynthesis of lipid A, an essential component of the outer membrane of Gram-negative bacteria UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + acetate
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 ?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O67648
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-alpha-D-glucosamine + H2O
-
 Aquifex aeolicus UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + acetate
-
 ?
UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-alpha-D-glucosamine + H2O the enzyme catalyzes the first committed step in the biosynthesis of lipid A, an essential component of the outer membrane of Gram-negative bacteria Aquifex aeolicus UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
LpxC
-
 Aquifex aeolicus