Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the metal bound to LpxC purified from Escherichia coli grown in minimal medium is mainly Fe(II). However, the ratio of iron/zinc bound to LpxC varies with the metal content of the medium. Fe(II) is the thermodynamically favored metal cofactor for LpxC under cellular conditions, but the metal cofactor in LpxC can switch between iron and zinc in response to perturbations in available metal ions | Escherichia coli | |
Zn2+ | the metal bound to LpxC purified from Escherichia coli grown in minimal medium is mainly Fe(II). However, the ratio of iron/zinc bound to LpxC varies with the metal content of the medium. The metal cofactor in LpxC can switch between iron and zinc in response to perturbations in available metal ions | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
pulldown method to rapidly purify tagged LpxC under anaerobic conditions | Escherichia coli |