Cloned (Comment) | Organism |
---|---|
overepression of LpxC variants in Escherichia coli | Escherichia coli |
overepression of LpxC variants in Escherichia coli | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
D105A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 56% of the specific activity of the wild-type extract | Aquifex aeolicus |
D105N | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 67% of the specific activity of the wild-type extract | Aquifex aeolicus |
D105S | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 78% of the specific activity of the wild-type extract | Aquifex aeolicus |
D242A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 9.5% of the specific activity of the wild-type extract | Escherichia coli |
D242Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.04% of the specific activity of the wild-type extract | Escherichia coli |
D246A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.001% of the specific activity of the wild-type extract. Contains 64% Fe2+ compared to wild-type Zn2+ content | Aquifex aeolicus |
D246A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.06% of the specific activity of the wild-type extract | Escherichia coli |
D246N | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 3.5% of the specific activity of the wild-type extract | Aquifex aeolicus |
D246S | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about less than 0.0005% of the specific activity of the wild-type extract | Aquifex aeolicus |
E100A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 72% of the specific activity of the wild-type extract | Aquifex aeolicus |
E100N | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 61% of the specific activity of the wild-type extract | Aquifex aeolicus |
E100S | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 83% of the specific activity of the wild-type extract | Aquifex aeolicus |
E234A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 4.8% of the specific activity of the wild-type extract | Aquifex aeolicus |
E234N | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about less than 0.0005% of the specific activity of the wild-type extract | Aquifex aeolicus |
E234S | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 6.7% of the specific activity of the wild-type extract | Aquifex aeolicus |
E78A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.1% of the specific activity of the wild-type extract | Escherichia coli |
E78A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 9.8% of the specific activity of the wild-type extract. Contains 28% Fe2+ compared to wild-type Zn2+ content. The specific activity of the E78A LpxC variant is neither inhibited nor activated by the addition of up to 1 mM Zn2+ | Aquifex aeolicus |
E78Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.03% of the specific activity of the wild-type extract | Escherichia coli |
E78Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about less than 0.0005% of the specific activity of the wild-type extract | Aquifex aeolicus |
H19A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.07% of the specific activity of the wild-type extract. | Escherichia coli |
H19A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 5% of the specific activity of the wild-type extract. Contains 51% Fe2+ compared to wild-type Zn2+ content | Aquifex aeolicus |
H19Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 1.1% of the specific activity of the wild-type extract | Escherichia coli |
H19Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 45% of the specific activity of the wild-type extract | Aquifex aeolicus |
H19Y | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.5% of the specific activity of the wild-type extract | Escherichia coli |
H19Y | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 4.4% of the specific activity of the wild-type extract | Aquifex aeolicus |
H238A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.09% of the specific activity of the wild-type extract | Escherichia coli |
H238A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.1% of the specific activity of the wild-type extract. Extract overexpressing H238A is stimulated approximately 20fold by the addition of ZnSO4 | Aquifex aeolicus |
H265A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.03% of the specific activity of the wild-type extract | Escherichia coli |
H265Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.02% of the specific activity of the wild-type extract | Escherichia coli |
H265Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about less than 0.0005% of the specific activity of the wild-type extract | Aquifex aeolicus |
H79A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.006% of the specific activity of the wild-type extract. Contains 10% Fe2+ compared to wild-type Zn2+ content. Extract overexpressing H79A is stimulated approximately 20fold by the addition of ZnSO4 | Aquifex aeolicus |
H79A | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.04% of the specific activity of the wild-type extract | Escherichia coli |
H79Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.008% of the specific activity of the wild-type extract | Aquifex aeolicus |
H79Q | expressed to level comparable to wild-type LpxC. The extract overexpressing LpxC exhibits about 0.08% of the specific activity of the wild-type extract | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the low activity of LpxC variants at positions H79 and H238, coupled with the ability of Zn2+ to stimulate the activity of these enzymes and the low Zn2+ content of the purified variant enzymes suggests that these residues directly coordinate a catalytic Zn2+ in LpxC. The variants with alanine substituted at H265 or D246 also exhibit large decreases in LpxC activity, suggesting that one of these residues may constitute a third protein ligand to the active-site Zn2+ | Escherichia coli | |
Zn2+ | wild-type enzyme contains 0.98 mg of Zn2+ per g of protein. The low activity of LpxC variants at positions H79 and H238, coupled with the ability of Zn2+ to stimulate the activity of these enzymes and the low Zn2+ content of the purified variant enzymes suggests that these residues directly coordinate a catalytic Zn2+ in LpxC. The variants with alanine substituted at H265 or D246 also exhibit large decreases in LpxC activity, suggesting that one of these residues may constitute a third protein ligand to the active-site Zn2+ | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | Escherichia coli | LpxC catalyzes a step in the biosynthesis of lipid A in Gram-negative bacteria | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | Aquifex aeolicus | LpxC catalyzes a step in the biosynthesis of lipid A in Gram-negative bacteria | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.84 | - |
- |
Escherichia coli |
0.84 | - |
- |
Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | - |
Escherichia coli | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | - |
Aquifex aeolicus | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | LpxC catalyzes a step in the biosynthesis of lipid A in Gram-negative bacteria | Escherichia coli | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | LpxC catalyzes a step in the biosynthesis of lipid A in Gram-negative bacteria | Aquifex aeolicus | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LpxC protein | - |
Escherichia coli |
LpxC protein | - |
Aquifex aeolicus |
UDP-(3-O-acyl)-N-acetylglucosamine deacetylase | - |
Escherichia coli |
UDP-(3-O-acyl)-N-acetylglucosamine deacetylase | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
60 | - |
assay at | Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
6 h, stable | Escherichia coli |
60 | - |
stable for up to 20 min | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Aquifex aeolicus |
5.9 | - |
assay at | Escherichia coli |