Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21 is used as host for expression of Strep-tagged LpxC protein | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | Escherichia coli | the enzyme is involved in lipid A biosynthesis | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | Escherichia coli W3110 / ATCC 27325 | the enzyme is involved in lipid A biosynthesis | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | - |
Escherichia coli | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | the enzyme is involved in lipid A biosynthesis | Escherichia coli | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | - |
Escherichia coli W3110 / ATCC 27325 | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? | |
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine + H2O | the enzyme is involved in lipid A biosynthesis | Escherichia coli W3110 / ATCC 27325 | UDP-3-O-((R)-3-hydroxymyristoyl)-D-glucosamine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LpxC protein | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the viability of Gram negative bacteria depends on lipid A, which is the anchoring component of lipopolysaccharides in the outer membrane. Lipopolysaccharides provides a permeability barrier to the cell and Escherichia coli mutants affected in lipopolysaccharide biosynthesis are hypersensitive to antibiotics and detergents. Lipid A is the immuno-effective portion of lipopolysaccharide causing a septic shock by Gram negative bacteria. Lipid A and the enzymes involved in lipopolysaccharide biosynthesis are considered to be attractive drug targets. Although LPS is essential, Escherichia coli is unable to tolerate elevated levels of it, which induces accumulation of abnormal membranes. The proper lipopolysaccharide amount depends on the cellular level of the UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase LpxC, the key enzyme in lipid A synthesis. The membrane-bound protease FtsH controls the level of LpxC via proteolysis. The C-terminus of LpxC contains a signal sequence necessary for FtsH-dependent degradation | Escherichia coli |