Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion method, native crystal structure and product complexes of SpPgdA | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
I419G | kcat/Km is 15fold lower than wild-type value | Streptococcus pneumoniae |
K304I | kcat/Km is 1.7fold higher than wild-type value | Streptococcus pneumoniae |
L302A | kcat/Km is 2.5fold higher than wild-type value | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | wild-type enzyme | Streptococcus pneumoniae | |
4 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | mutant enzyme L302A | Streptococcus pneumoniae | |
6.2 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | mutant enzyme K304I | Streptococcus pneumoniae | |
26 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | mutant enzyme I419G | Streptococcus pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.05 mM, 30fold increase in activity | Streptococcus pneumoniae | |
additional information | SpPgdA is a metalloenzyme using a His-His-Asp zinc-binding triad with a nearby aspartic acid and histidine acting as the catalytic base and acid, respectively | Streptococcus pneumoniae | |
Zn2+ | 0.05 mM, 5.5fold increase in activity | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated peptidoglycan + H2O | Streptococcus pneumoniae | peptidoglycan GlcNAc deacetylase protects the Gram-positive bacterial cell wall from host lysozymes by deacetylating peptidoglycan GlcNAc residues | deacetylated peptidoglycan + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q8DP63 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated peptidoglycan + H2O | - |
Streptococcus pneumoniae | deacetylated peptidoglycan + acetate | - |
? | |
acetylated peptidoglycan + H2O | peptidoglycan GlcNAc deacetylase protects the Gram-positive bacterial cell wall from host lysozymes by deacetylating peptidoglycan GlcNAc residues | Streptococcus pneumoniae | deacetylated peptidoglycan + acetate | - |
? | |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | - |
Streptococcus pneumoniae | GlcNAc-beta-1,4-GlcN-beta-1,4-GlcNAc + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidoglycan GlcNAc deacetylase | - |
Streptococcus pneumoniae |
SpPgdA | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | mutant enzyme I419G | Streptococcus pneumoniae | |
0.55 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | wild-type enzyme | Streptococcus pneumoniae | |
1.5 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | mutant enzyme L302A | Streptococcus pneumoniae | |
1.6 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | mutant enzyme K304I | Streptococcus pneumoniae |