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Literature summary for 3.5.1.104 extracted from

  • Psylinakis, E.; Boneca, I.G.; Mavromatis, K.; Deli, A.; Hayhurst, E.; Foster, S.J.; Varum, K.M.; Bouriotis, V.
    Peptidoglycan N-Acetylglucosamine Deacetylases from Bacillus cereus, Highly Conserved Proteins in Bacillus anthracis (2005), J. Biol. Chem., 280, 30856-30863.
    View publication on PubMed
Search for more literature for 3.5.1.104

Cloned(Commentary)

Cloned (Comment) Organism
the gene bc1960 is cloned and expressed in Escherichia coli Bacillus cereus
the gene bc3618 is cloned and expressed in Escherichia coli Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
CuCl2
-
 Bacillus cereus
ZnCl2
-
 Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc enzyme BC1960 Bacillus cereus
0.37
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc enzyme BC1960 Bacillus cereus
0.45
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc
-
 Bacillus cereus
0.5
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc
-
 Bacillus cereus
1.18
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc enzyme BC1960 Bacillus cereus
1.5
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc
-
 Bacillus cereus
2.2
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc
-
 Bacillus cereus
2.46
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc enzyme BC1960 Bacillus cereus
3.9
-
 GlcNAc-beta-1,4-GlcNAc
-
 Bacillus cereus
4.1
-
 GlcNAc-beta-1,4-GlcNAc enzyme BC1960 Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 10% increase in activity Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylated peptidoglycan + H2O Bacillus cereus enzymatic deacetylation of chemically acetylated vegetative peptidoglycan from Bacillus cereus by BC1960 and BC3618 results in increased resistance to lysozyme digestion deacetylated peptidoglycan + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylated peptidoglycan + H2O enzymatic deacetylation of chemically acetylated vegetative peptidoglycan from Bacillus cereus by BC1960 and BC3618 results in increased resistance to lysozyme digestion Bacillus cereus deacetylated peptidoglycan + acetate
-
 ?
acetylated peptidoglycan + H2O effective in deacetylating cell wall peptidoglycan from the Gram(+) Bacillus cereus and Bacillus subtilis and the Gram(-) Helicobacter pylori Bacillus cereus deacetylated peptidoglycan + acetate
-
 ?
GlcNAc-beta-1,4-GlcNAc + H2O no deacetylation of the reducing GlcNAc residue Bacillus cereus ?
-
 ?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O deacetylates all GlcNAc residues of the oligomer except the reducing end ones Bacillus cereus ?
-
 ?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc is the favorable substrate. Deacetylates all GlcNAc residues of the oligomer except the reducing end ones Bacillus cereus ?
-
 ?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O deacetylates all GlcNAc residues of the oligomer except the reducing end ones Bacillus cereus ?
-
 ?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O deacetylates all GlcNAc residues of the oligomer except the reducing end ones Bacillus cereus ?
-
 ?
additional information no activity with N-acetyl-D-glucosamine Bacillus cereus ?
-
 ?

Synonyms

Synonyms Comment Organism
enzyme BC1960
-
 Bacillus cereus
enzyme BC3618
-
 Bacillus cereus
peptidoglycan N-acetylglucosamine deacetylase
-
 Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 enzyme BC3618 Bacillus cereus
50
-
 enzyme BC1960 Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 enzyme BC1960 retains 95% of its activity after 24 h Bacillus cereus
50
-
 enzyme BC3618 is inactivated after 1h Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 enzyme BC1960 Bacillus cereus
8
-
 enzyme BC3618 Bacillus cereus