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Literature summary for 3.5.1.10 extracted from

  • Nagy, P.L.; Marolewski, A.; Benkovic, S.J.; Zalkin, H.
    Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli (1995), J. Bacteriol., 3, 1292-1298.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
methionine
-
Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
purU gene cloned into overexpression vector pT7-7, pT7-PU1 tranformed into Escherichia coli BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
glycine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
10-formyltetrahydrofolate 2.0 mM methionine added Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31800
-
calculated from the sequence of the cloned gene Escherichia coli
32000
-
6 * 32000, SDS-PAGE Escherichia coli
190000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P37051
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.174
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + H2O
-
Escherichia coli formate + tetrahydrofolate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 32000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
formyl-FH4 hydrolase
-
Escherichia coli
formyltetrahydrofolate hydrolase
-
Escherichia coli