Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methionine | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
purU gene cloned into overexpression vector pT7-7, pT7-PU1 tranformed into Escherichia coli BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
10-formyltetrahydrofolate | 2.0 mM methionine added | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31800 | - |
calculated from the sequence of the cloned gene | Escherichia coli |
32000 | - |
6 * 32000, SDS-PAGE | Escherichia coli |
190000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P37051 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.174 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + H2O | - |
Escherichia coli | formate + tetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 32000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
formyl-FH4 hydrolase | - |
Escherichia coli |
formyltetrahydrofolate hydrolase | - |
Escherichia coli |