Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N24S | mutant shows completely preserved asparaginase and glutaminase activities, long-term storage stability, improved thermal parameters, and good resistance to proteases derived from leukaemia cells. The mutant displays a modification in the hydrogen bond network related to residue 24, and a general rigidification of the monomer as compared to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-asparagine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.03 | - |
L-asparagine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
3.95 | - |
L-glutamine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.14 | - |
L-glutamine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00805 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
103 | - |
mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
105 | - |
wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | reaction of EC 3.5.1.2 | Escherichia coli | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AnsB | - |
Escherichia coli |
L-asparaginase 2 | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
melting temperature, wild-type | Escherichia coli |
59 | - |
melting temperature, mutant N24S | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
L-glutamine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.53 | - |
L-glutamine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
58.8 | - |
L-asparagine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
59.8 | - |
L-asparagine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-glutamine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.13 | - |
L-glutamine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1782 | - |
L-asparagine | mutant N24S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1975 | - |
L-asparagine | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |