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Literature summary for 3.5.1.1 extracted from

  • Maggi, M.; Chiarelli, L.R.; Valentini, G.; Scotti, C.
    Engineering of Helicobacter pylori L-asparaginase characterization of two functionally distinct groups of mutants (2015), PLoS ONE, 10, e0117025 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.1

Protein Variants

Protein Variants Comment Organism
M121C about 2.5fold reduced activtiy Helicobacter pylori
M121C/T169M mutant has a preserved efficiency vs L-asparagine but is completely unable to carry out L-glutamine hydrolysis. The mutant does not exert any cytotoxic effect on HL-60 cells Helicobacter pylori
Q63E mutant displays similar catalytic efficiency versus asparagine and halved glutaminase efficiency with respect to the wild type enzyme, is able to exert a cytotoxic effect comparable to, or higher than, the one of the wild type enzyme Helicobacter pylori
T169M about 4fold reduced catalytic activity Helicobacter pylori
T16D replacement of catalytic threonine, depletes the enzyme of its catalytic activities with L-asparagine and L-glutamine Helicobacter pylori
T95D replacement of catalytic threonine, depletes the enzyme of its catalytic activities with L-asparagine and L-glutamine Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 L-asparagine mutant Q63E, pH 7.5, 37°C Helicobacter pylori
0.23
-
 L-asparagine mutant M121C/T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.7, pH 7.5, 37°C Helicobacter pylori
0.25
-
 L-asparagine mutant M121C, cooperative kinetic toward L-Asn, Hill coefficient 1.5, pH 7.5, 37°C Helicobacter pylori
0.25
-
 L-asparagine mutant T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.8, pH 7.5, 37°C Helicobacter pylori
0.29
-
 L-asparagine wild-type, pH 7.5, 37°C Helicobacter pylori
46.4
-
 L-glutamine wild-type, cooperative kinetic toward L-Gln, Hill coefficient 2.0, pH 7.5, 37°C Helicobacter pylori
76.5
-
 L-glutamine mutant Q63E, cooperative kinetic toward L-Gln, Hill coefficient 2.3, pH 7.5, 37°C Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori A0A2X5A091
-
-
Helicobacter pylori ATCC 43504 A0A2X5A091
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Helicobacter pylori L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Helicobacter pylori ATCC 43504 L-aspartate + NH3
-
 ?
L-glutamine + H2O reaction of EC 3.5.1.2 Helicobacter pylori L-glutamate + NH3
-
 ?
L-glutamine + H2O reaction of EC 3.5.1.2 Helicobacter pylori ATCC 43504 L-glutamate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
AnsB
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 L-glutamine wild-type, pH 7.5, 37°C Helicobacter pylori
4.83
-
 L-asparagine mutant M121C, pH 7.5, 37°C Helicobacter pylori
6.53
-
 L-asparagine mutant Q63E, pH 7.5, 37°C Helicobacter pylori
7.4
-
 L-asparagine mutant T169M, pH 7.5, 37°C Helicobacter pylori
13.95
-
 L-asparagine mutant M121C/T169M, pH 7.5, 37°C Helicobacter pylori
14.5
-
 L-glutamine mutant Q63E, pH 7.5, 37°C Helicobacter pylori
19.2
-
 L-asparagine wild-type, pH 7.5, 37°C Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 L-glutamine mutant Q63E, cooperative kinetic toward L-Gln, Hill coefficient 2.3, pH 7.5, 37°C Helicobacter pylori
0.47
-
 L-glutamine wild-type, cooperative kinetic toward L-Gln, Hill coefficient 2.0, pH 7.5, 37°C Helicobacter pylori
29.6
-
 L-asparagine mutant T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.8, pH 7.5, 37°C Helicobacter pylori
30.2
-
 L-asparagine mutant M121C, cooperative kinetic toward L-Asn, Hill coefficient 1.5, pH 7.5, 37°C Helicobacter pylori
60.7
-
 L-asparagine mutant M121C/T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.7, pH 7.5, 37°C Helicobacter pylori
65.3
-
 L-asparagine mutant Q63E, pH 7.5, 37°C Helicobacter pylori
66.4
-
 L-asparagine wild-type, pH 7.5, 37°C Helicobacter pylori