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Literature summary for 3.5.1.1 extracted from

  • El-Sharkawy, A.; Farag, A.; Embaby, A.; Saeed, H.; El-Shenawy, M.
    Cloning, expression and characterization of aeruginosa EGYII L-asparaginase from Pseudomonas aeruginosa strain EGYII DSM 101801 in E.coli BL21(DE3) pLysS (2016), J. Mol. Catal. B, 132, 16-23 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, 113% of initial activity Pseudomonas aeruginosa
EDTA 1 mM, 105% of initial activity, 5 mM Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 1 mM, 109% of initial activity, 5 mM, 86% of initial activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
63.3
-
L-asparagine 37°C, pH 7.5 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM, 2fold activation Pseudomonas aeruginosa
Zn2+ 1 mM, 109% of initial activity, 5 mM, 86% of initial activity Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa A0A0U3U5E8
-
-
Pseudomonas aeruginosa DSM 101801 A0A0U3U5E8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1477
-
37°C, pH 7.5 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
Pseudomonas aeruginosa L-aspartate + NH3
-
?
L-asparagine + H2O
-
Pseudomonas aeruginosa DSM 101801 L-aspartate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
20 min, almost 80% residual activity, 30 min, almost 60% residual activity Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
Pseudomonas aeruginosa