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Literature summary for 3.5.1.1 extracted from
- Partial purification and characterization of L-asparaginase from an endophytic Talaromyces pinophilus isolated from the rhizomes of Curcuma amada (2016), J. Mol. Catal. B, 124, 83-91 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 0.5 mM, 12% increase in activity | Talaromyces pinophilus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 90% residual activity | Talaromyces pinophilus | |
| Cu2+ | 10 mM, 92% residual activity | Talaromyces pinophilus | |
| SDS | 10 mM, 30% residual activity | Talaromyces pinophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.4 | - |
L-asparagine | pH 8.0, 37°C | Talaromyces pinophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 101000 | - |
PAGE | Talaromyces pinophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces pinophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Talaromyces pinophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23.7 | - |
substrate L-glutamine, pH 8.0, 37°C | Talaromyces pinophilus |
| 145 | - |
substrate L-asparagine, pH 8.0, 37°C | Talaromyces pinophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Talaromyces pinophilus | L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | reaction of EC 3.5.1.2 | Talaromyces pinophilus | L-glutamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 62000 + 1 * 39000, SDS-PAGE | Talaromyces pinophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Talaromyces pinophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | 37 | - |
Talaromyces pinophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 286.3 | - |
L-asparagine | pH 8.0, 37°C | Talaromyces pinophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Talaromyces pinophilus |
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