Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 16.5% of initial activity | Priestia megaterium | |
Co2+ | 10 mM, 35.6% of initial activity | Priestia megaterium | |
Cu2+ | 10 mM, 22.3% of initial activity | Priestia megaterium | |
Fe2+ | 10 mM, 13.3% of initial activity | Priestia megaterium | |
Hg2+ | 10 mM, 23% of initial activity | Priestia megaterium | |
Mg2+ | 10 mM, 3.3% of initial activity | Priestia megaterium | |
Mn2+ | 10 mM, 20.5% of initial activity | Priestia megaterium | |
SDS | 2 mM, 3.1% of initial activity | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-asparagine | 37°C, pH 8.5 | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | 10 mM, 110% of initial activity | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
185000 | - |
gel filtration | Priestia megaterium |
185000 | - |
PAGE | Priestia megaterium |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | 50% , 78% residual activity | Priestia megaterium |
Ethanol | 50% , 79% residual activity | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium MG1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
215 | - |
37°C, pH 8.5 | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Priestia megaterium | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Priestia megaterium MG1 | L-aspartate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 47000, SDS-PAGE | Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Priestia megaterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | more than 40% of maximum activity within | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, at least 40% residual activity | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Priestia megaterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | more than 50% of maximum activity within | Priestia megaterium |