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Literature summary for 3.5.1.1 extracted from

  • Lu, X.; Chen, J.; Jiao, L.; Zhong, L.; Lu, Z.; Zhang, C.; Lu, F.
    Improvement of the activity of L-asparaginase I improvement of the catalytic activity of L-asparaginase I from Bacillus megaterium H-1 by in vitro directed evolution (2019), J. Biosci. Bioeng., 128, 683-689 .
    View publication on PubMed
Search for more literature for 3.5.1.1

Application

Application Comment Organism
food industry approximately 88.5% (0.978 mg/kg) acrylamide can be removed from fried potato chips by mutant V26A/E30G/D181G/V245G/G276D pre-treatment Priestia megaterium

Protein Variants

Protein Variants Comment Organism
V26A/E30G/D181G/V245G/G276D 21fold increase in catalytic efficiency, mutant shows tolerance toward wider range of pH values and higher temperatures than its wild-type counterpart Priestia megaterium
V26A/E30G/K122N/G276D 20fold increase in catalytic efficiency Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21.6
-
 L-asparagine mutant V26A/E30G/D181G/V245G/G276D, pH 8.0, 40°C Priestia megaterium
26.1
-
 L-asparagine mutant V26A/E30G/K122N/G276D, pH 8.0, 40°C Priestia megaterium
28.6
-
 L-asparagine wild-type, pH 8.0, 40°C Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium H-1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55.7
-
 wild-type, pH 8.0, 40°C Priestia megaterium
1017
-
 mutant V26A/E30G/K122N/G276D, pH 8.0, 40°C Priestia megaterium
1146
-
 mutant V26A/E30G/D181G/V245G/G276D, pH 8.0, 40°C Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Priestia megaterium L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Priestia megaterium H-1 L-aspartate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
AnsA
-
 Priestia megaterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.62
-
 L-asparagine wild-type, pH 8.0, 40°C Priestia megaterium
52.8
-
 L-asparagine mutant V26A/E30G/D181G/V245G/G276D, pH 8.0, 40°C Priestia megaterium
57.7
-
 L-asparagine mutant V26A/E30G/K122N/G276D, pH 8.0, 40°C Priestia megaterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.055
-
 L-asparagine wild-type, pH 8.0, 40°C Priestia megaterium
2.21
-
 L-asparagine mutant V26A/E30G/K122N/G276D, pH 8.0, 40°C Priestia megaterium
2.44
-
 L-asparagine mutant V26A/E30G/D181G/V245G/G276D, pH 8.0, 40°C Priestia megaterium