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Literature summary for 3.5.1.1 extracted from
- Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme II (2015), Enzyme Microb. Technol., 79-80, 49-54 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | recombinant asparaginase isozyme AnsB fused with the pelB signal sequence and a five aspartate tag is secreted efficiently into culture medium at 34.6 U/mg cell of specific activity. By batch fermentation, recombinant Escherichia coli produces 40.8 U/ml asparaginase isozyme II in the medium. Deletion of the gspDE gene reduces extracellular production of asparaginase isozyme II | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P00805 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AnsB | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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