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Literature summary for 3.5.1.1 extracted from

  • Badoei-Dalfard, A.
    Purification and characterization of L-asparaginase from Pseudomonas aeruginosa strain SN004 Production optimization by statistical methods (2015), Biocatal. Agricult. Biotechnol., 4, 388-397 .
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Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, 64% of initial activity Pseudomonas aeruginosa
Co2+ 2 mM, 67% of initial activity Pseudomonas aeruginosa
Hg2+ 2 mM, 2% of initial activity Pseudomonas aeruginosa
K+ 2 mM, 69% of initial activity Pseudomonas aeruginosa
SDS 2 mM, 30% of initial activity Pseudomonas aeruginosa
Zn2+ 2 mM, 19% of initial activity Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 2 mM, 114% of initial activity Pseudomonas aeruginosa
Mg2+ 2 mM, 116% of initial activity Pseudomonas aeruginosa
Mn2+ 2 mM, 112% of initial activity Pseudomonas aeruginosa
Na+ 2 mM, 113% of initial activity Pseudomonas aeruginosa
Triton X-100 2 mM, 114% of initial activity Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa SN004
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7
-
 Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa the maximum amount of L-asparaginase produced is 785U/ml from the optimized medium containing L-asparagine (0.5%), glucose (0.2%), NaCl (0.045%)and K2HPO4 (0.045%) up