Literature summary for 3.5.1.1 extracted from

Mohan Kumar, N.S.; Manonmani, H.K.
Purification, characterization and kinetic properties of extracellular L-asparaginase produced by Cladosporium sp (2013), World J. Microbiol. Biotechnol., 29, 577-587.

Search for more literature for 3.5.1.1

Application

Application	Comment	Organism
food industry	the enzyme reduces acrylamide content in starchy fried food commodities	Cladosporium sp.

General Stability

General Stability	Organism
in-vitro half-life of purified L-asparaginase in mammalian blood serum is 93.69 h	Cladosporium sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	-	Cladosporium sp.
Ca2+	-	Cladosporium sp.
Co2+	-	Cladosporium sp.
Cu2+	-	Cladosporium sp.
K+	-	Cladosporium sp.
Mg2+	-	Cladosporium sp.
Na+	-	Cladosporium sp.
Zn2+	-	Cladosporium sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
100	-	L-asparagine	at pH 6.3 and 30°C	Cladosporium sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe3+	stimulates activity	Cladosporium sp.
KI	stimulates activity	Cladosporium sp.
Pb2+	stimulates activity	Cladosporium sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	2 * 37000 + 1 * 47000, SDS-PAGE	Cladosporium sp.
47000	-	2 * 37000 + 1 * 47000, SDS-PAGE	Cladosporium sp.
120000	-	gel filtration	Cladosporium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-asparagine + H2O	Cladosporium sp.	substrate hydrolysis efficiency of L-asparagine is 8fold higher than that of L-glutamine	L-aspartate + NH3	-	?
L-glutamine + H2O	Cladosporium sp.	-	L-glutamate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Cladosporium sp.	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:wheat bran-grown cell	-	Cladosporium sp.	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-asparagine + H2O	substrate hydrolysis efficiency of L-asparagine is 8fold higher than that of L-glutamine	Cladosporium sp.	L-aspartate + NH3	-	?
L-glutamine + H2O	-	Cladosporium sp.	L-glutamate + NH3	-	?

Subunits

Subunits	Comment	Organism
heterotrimer	2 * 37000 + 1 * 47000, SDS-PAGE	Cladosporium sp.

Synonyms

Synonyms	Comment	Organism
L-asparaginase	-	Cladosporium sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Cladosporium sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.3	-	-	Cladosporium sp.

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Release 2023.1 (January 2023)