Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
M121C | mutants has nearly 2.5fold reduced catalytic activity compared to the wild type enzyme | Helicobacter pylori |
M121C/T169M | the mutant has a preserved efficiency versus L-asparagine but is completely unable to carry out L-glutamine hydrolysis | Helicobacter pylori |
Q63E | the mutant endows with a similar catalytic efficiency versus L-asparagine and halved glutaminase efficiency with respect to the wild type enzyme | Helicobacter pylori |
T169M | mutants has nearly 4fold reduced catalytic activity compared to the wild type enzyme | Helicobacter pylori |
T16D | inactive | Helicobacter pylori |
T95E | inactive | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Helicobacter pylori | strongly preferred substrate | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Helicobacter pylori CCUG 17874 | strongly preferred substrate | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | Helicobacter pylori | - |
L-glutamic acid + NH3 | - |
? | |
L-glutamine + H2O | Helicobacter pylori CCUG 17874 | - |
L-glutamic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | I0ZIE0 | - |
- |
Helicobacter pylori CCUG 17874 | I0ZIE0 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex S200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | strongly preferred substrate | Helicobacter pylori | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | strongly preferred substrate | Helicobacter pylori CCUG 17874 | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | - |
Helicobacter pylori | L-glutamic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Helicobacter pylori CCUG 17874 | L-glutamic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
ASNase | - |
Helicobacter pylori |
HPA | - |
Helicobacter pylori |
L-ASNase | - |
Helicobacter pylori |
type II L-asparaginase | - |
Helicobacter pylori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
the wild type enzyme shows 50% activity after 10 min incubation at 53°C | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.83 | - |
L-asparagine | mutant enzyme M121C, at pH 7.5 and 37°C | Helicobacter pylori | |
6.53 | - |
L-asparagine | mutant enzyme Q63E, at pH 7.5 and 37°C | Helicobacter pylori | |
7.4 | - |
L-asparagine | mutant enzyme T169M, at pH 7.5 and 37°C | Helicobacter pylori | |
13.95 | - |
L-asparagine | mutant enzyme M121C/T169M, at pH 7.5 and 37°C | Helicobacter pylori | |
14.5 | - |
L-glutamine | mutant enzyme Q63E, at pH 7.5 and 37°C | Helicobacter pylori | |
19.26 | - |
L-asparagine | wild type enzyme, at pH 7.5 and 37°C | Helicobacter pylori | |
22.1 | - |
L-glutamine | wild type enzyme, at pH 7.5 and 37°C | Helicobacter pylori |