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Literature summary for 3.5.1.1 extracted from

  • Nomme, J.; Su, Y.; Lavie, A.
    Elucidation of the specific function of the conserved threonine triad responsible for human L-asparaginase autocleavage and substrate hydrolysis (2014), J. Mol. Biol., 426, 2471-2485.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
glycine the enzyme is activated by incubating for 4 days in a 2.0 M glycine (pH 9.0) solution at room temperature Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) C41 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 2.2-2.5 M sodium malonate (pH 7.0) Homo sapiens

Protein Variants

Protein Variants Comment Organism
T168S the mutant shows no self-cleavage Homo sapiens
T186V the inactive mutant shows partial (45%) self-cleavage Homo sapiens
T219A the inactive mutant shows a complete, albeit slow self-cleavage Homo sapiens
T219V the inactive mutant displays partial (50%) self-cleavage Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 beta-L-Asp-L-Phe wild type enzyme, at pH 7.5 and 37°C Homo sapiens
2.09
-
 L-asparagine wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE Homo sapiens
20000
-
 1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE Homo sapiens
37000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Homo sapiens
-
 L-aspartate + NH3
-
 ?
additional information Homo sapiens to become enzymatically active, ASNase3 must undergo autocleavage between residues Gly167 and Thr168 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q7L266
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-L-Asp-L-Phe + H2O
-
 Homo sapiens ?
-
 ?
L-asparagine + H2O
-
 Homo sapiens L-aspartate + NH3
-
 ?
additional information to become enzymatically active, ASNase3 must undergo autocleavage between residues Gly167 and Thr168 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ASNase3
-
 Homo sapiens
L-asparaginase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 beta-L-Asp-L-Phe wild type enzyme, at pH 7.5 and 37°C Homo sapiens
3.19
-
 L-asparagine wild type enzyme, at pH 7.5 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.52
-
 L-asparagine wild type enzyme, at pH 7.5 and 37°C Homo sapiens
6.66
-
 beta-L-Asp-L-Phe wild type enzyme, at pH 7.5 and 37°C Homo sapiens