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Literature summary for 3.5.1.1 extracted from

  • Borek, D.; Kozak, M.; Pei, J.; Jaskolski, M.
    Crystal structure of active site mutant of antileukemic L-asparaginase reveals conserved zinc-binding site (2014), FEBS J., 281, 4097-4111.
    View publication on PubMed
Search for more literature for 3.5.1.1

Application

Application Comment Organism
medicine the enzyme is an important drug in the treatment of childhood acute lymphoblastic leukemia Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme D90E, hanging drop vapor diffusion method, using 25% PEG MME 550, 100 mM MES pH 6.5, 10 mM ZnSO4 or 30% (w/v) PEG MME 550, 100 mM bicine pH 9.0, 100 mM NaCl or 100 mM HEPES pH 7.5, saturated solution of tribasic sodium citrate mixed with buffer, at citrate:buffer ratio of 9:1 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D90E active site mutation Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Escherichia coli
-
 L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00805
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Escherichia coli L-aspartate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
L-asparaginase type II
-
 Escherichia coli