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Literature summary for 3.5.1.1 extracted from

  • Meena, B.; Anburajan, L.; Dheenan, P.S.; Begum, M.; Vinithkumar, N.V.; Dharani, G.; Kirubagaran, R.
    Novel glutaminase free L-asparaginase from Nocardiopsis alba NIOT-VKMA08: production, optimization, functional and molecular characterization (2015), Bioprocess Biosyst. Eng., 38, 373-388.
    View publication on PubMed
Search for more literature for 3.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
additional information not influenced by Tween-20 Nocardiopsis alba
potassium iodide 231.68% activity at 1 mM Nocardiopsis alba
Triton X-100 161.26% activity at 1% (v/v) Nocardiopsis alba
Tween-80 123.45% activity at 1% (v/v) Nocardiopsis alba

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Nocardiopsis alba

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol complete inhibition at 2 mM Nocardiopsis alba
Ca2+ 23.69% residual activity at 1 mM Nocardiopsis alba
Cd2+ complete inhibition at 1 mM Nocardiopsis alba
Co2+ 91.24% residual activity at 1 mM Nocardiopsis alba
Cu2+ complete inhibition at 1 mM Nocardiopsis alba
cysteine complete inhibition at 1 mM Nocardiopsis alba
Hg2+ complete inhibition at 1 mM Nocardiopsis alba
iodoacetamide complete inhibition at 1 mM Nocardiopsis alba
Mg2+ 29.47% residual activity at 1 mM Nocardiopsis alba
Mn2+ 31.56% residual activity at 1 mM Nocardiopsis alba
additional information urea and EDTA do not reveal any inhibitory effects on enzyme activity Nocardiopsis alba
SDS 33.21% residual activity at 2% (w/v) Nocardiopsis alba
Zn2+ 10.43% residual activity at 1 mM Nocardiopsis alba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.127
-
 L-asparagine at pH 8.0 and 37°C Nocardiopsis alba

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 145.97% activity at 1 mM Nocardiopsis alba
K+ 123.41% activity at 1 mM Nocardiopsis alba
Na+ 128.21% activity at 1 mM Nocardiopsis alba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Nocardiopsis alba highly specific substrate L-aspartate + NH3
-
 ?
L-asparagine + H2O Nocardiopsis alba NIOT-VKMA08 highly specific substrate L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Nocardiopsis alba W0KM71
-
-
Nocardiopsis alba NIOT-VKMA08 W0KM71
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by ammonium sulfate precipitation and Q-Sepharose column chromatography Nocardiopsis alba

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
 crude enzyme, at pH 8.0 and 37°C Nocardiopsis alba
93.28
-
 after 58.44fold purification, at pH 8.0 and 37°C Nocardiopsis alba

Storage Stability

Storage Stability Organism
-20°C, 3 weeks, lyophilized enzyme, no loss of activity Nocardiopsis alba
22°C, 24 h, lyophilized enzyme, no loss of activity Nocardiopsis alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O highly specific substrate Nocardiopsis alba L-aspartate + NH3
-
 ?
L-asparagine + H2O highly specific substrate Nocardiopsis alba NIOT-VKMA08 L-aspartate + NH3
-
 ?
additional information no activity with L-glutamine, L-aspartic acid, L-glutamic acid, thiourea, L-histidine, glutathione, L-arginine, and glycine Nocardiopsis alba ?
-
 ?
additional information no activity with L-glutamine, L-aspartic acid, L-glutamic acid, thiourea, L-histidine, glutathione, L-arginine, and glycine Nocardiopsis alba NIOT-VKMA08 ?
-
 ?

Synonyms

Synonyms Comment Organism
AnsA
-
 Nocardiopsis alba
L-asparaginase
-
 Nocardiopsis alba
L-asparagine amidohydrolase
-
 Nocardiopsis alba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Nocardiopsis alba

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75 the enzyme is active between 20 and 75°C. At temperatures above 37°C, reaction rate declines sharply with the loss of 80% of its original activity at 75°C Nocardiopsis alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Nocardiopsis alba

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 the enzyme is active between pH 6.5 and 10.0, while at lower pH, enzyme activity is decreasing (20%). There is more than 80% of maximum activity between pH 7.0 and 9.0 Nocardiopsis alba

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 the enzyme preserves more than 90% of its original activity when incubated up to 36 h at pH 8.0-10.0 Nocardiopsis alba