Application | Comment | Organism |
---|---|---|
food industry | reduction of acrylamide level in biscuits and bread | Rhizomucor miehei |
medicine | the recombinant enzyme potentiate a lectin's induction of leukemic K562 cell apoptosis | Rhizomucor miehei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhizomucor miehei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 55% residual activity at 2 mM | Rhizomucor miehei | |
Ag+ | 42% residual activity at 2 mM | Rhizomucor miehei | |
Ba2+ | 30% residual activity at 2 mM | Rhizomucor miehei | |
Co2+ | 69% residual activity at 2 mM | Rhizomucor miehei | |
Cr2+ | 37% residual activity at 2 mM | Rhizomucor miehei | |
Cu2+ | 13% residual activity at 2 mM | Rhizomucor miehei | |
Fe3+ | 41% residual activity at 2 mM | Rhizomucor miehei | |
Hg2+ | 24% residual activity at 2 mM | Rhizomucor miehei | |
K+ | 92% residual activity at 2 mM | Rhizomucor miehei | |
Mg2+ | 28% residual activity at 2 mM | Rhizomucor miehei | |
Na+ | 91% residual activity at 2 mM | Rhizomucor miehei | |
Ni2+ | 85% residual activity at 2 mM | Rhizomucor miehei | |
SDS | 56% residual activity at 2 mM | Rhizomucor miehei | |
Sn2+ | 87% residual activity at 2 mM | Rhizomucor miehei | |
Zn2+ | 17% residual activity at 2 mM | Rhizomucor miehei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 144% activity at 2 mM | Rhizomucor miehei | |
Mn2+ | 183% activity at 2 mM | Rhizomucor miehei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
2 * 72000, SDS-PAGE | Rhizomucor miehei |
75316 | - |
2 * 75316, calculated from amino acid sequence | Rhizomucor miehei |
133700 | - |
gel filtration | Rhizomucor miehei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Rhizomucor miehei | highest specificity for L-asparagine | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Rhizomucor miehei CAU432 | highest specificity for L-asparagine | L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor miehei | W0G253 | - |
- |
Rhizomucor miehei CAU432 | W0G253 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-IDA column chromatography | Rhizomucor miehei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7797 | - |
crude enzyme, at 45°C in 50 mM phosphate buffer (pH 7.0) | Rhizomucor miehei |
1.985 | - |
after 2.6fold purification, at 45°C in 50 mM phosphate buffer (pH 7.0) | Rhizomucor miehei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-asparagine + H2O | negligible activity | Rhizomucor miehei | D-aspartate + NH3 | - |
? | |
D-asparagine + H2O | negligible activity | Rhizomucor miehei CAU432 | D-aspartate + NH3 | - |
? | |
L-asparagine + H2O | highest specificity for L-asparagine | Rhizomucor miehei | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | highest specificity for L-asparagine | Rhizomucor miehei CAU432 | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | negligible activity | Rhizomucor miehei | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | negligible activity | Rhizomucor miehei CAU432 | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 72000, SDS-PAGE | Rhizomucor miehei |
homodimer | 2 * 75316, calculated from amino acid sequence | Rhizomucor miehei |
Synonyms | Comment | Organism |
---|---|---|
ASNase | - |
Rhizomucor miehei |
L-asparaginase | - |
Rhizomucor miehei |
L-asparagine amidohydrolase | - |
Rhizomucor miehei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rhizomucor miehei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | more than 50% activity between 30 and 65°C | Rhizomucor miehei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the recombinant enzyme is stable at 45°C for 30 min | Rhizomucor miehei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
676 | - |
L-asparagine | at pH 7.0 and 45°C | Rhizomucor miehei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhizomucor miehei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | more than 80% activity between pH 4.0 and 8.0 | Rhizomucor miehei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhizomucor miehei | calculated from amino acid sequence | - |
6.8 |