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Literature summary for 3.5.1.1 extracted from

  • Sudhir, A.P.; Dave, B.R.; Prajapati, A.S.; Panchal, K.; Patel, D.; Subramanian, R.B.
    Characterization of a recombinant glutaminase-free L-asparaginase (ansA3) enzyme with high catalytic activity from Bacillus licheniformis (2014), Appl. Biochem. Biotechnol., 174, 2504-2515.
    View publication on PubMed
Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 70% residual activity at 5 mM Bacillus licheniformis
EDTA less than 60% residual activity at 5 mM Bacillus licheniformis
Fe3+ less than 60% residual activity at 5 mM Bacillus licheniformis
Hg2+ complete inhibition at 5 mM Bacillus licheniformis
K+ less than 60% residual activity at 5 mM Bacillus licheniformis
Ni2+ about 85% residual activity at 5 mM Bacillus licheniformis
Urea less than 60% residual activity at 5 mM Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.671
-
 L-asparagine at 37°C and pH 8.0 Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slightly increased activity at 5 mM Bacillus licheniformis
Mn2+ slightly increased activity at 5 mM Bacillus licheniformis
Na+ slightly increased activity at 5 mM Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 1 * 37000, SDS-PAGE Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Bacillus licheniformis 100% activity L-aspartate + NH3
-
 ?
L-asparagine + H2O Bacillus licheniformis MTCC 429 100% activity L-aspartate + NH3
-
 ?
L-aspartic acid + NH3 Bacillus licheniformis less than 3% activity compared to L-asparagine ?
-
 ?
L-aspartic acid + NH3 Bacillus licheniformis MTCC 429 less than 3% activity compared to L-asparagine ?
-
 ?
L-glutamine + H2O Bacillus licheniformis less than 1% activity compared to L-asparagine L-glutamate + NH3
-
 ?
L-glutamine + H2O Bacillus licheniformis MTCC 429 less than 1% activity compared to L-asparagine L-glutamate + NH3
-
 ?
additional information Bacillus licheniformis no activity with D-asparagine monohydrate and L-glutamic acid ?
-
 ?
additional information Bacillus licheniformis MTCC 429 no activity with D-asparagine monohydrate and L-glutamic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis R4L284
-
-
Bacillus licheniformis MTCC 429 R4L284
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus licheniformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
135
-
 crude extract, at 37°C and pH 8.0 Bacillus licheniformis
407.6
-
 after 3.02fold purification, at 37°C and pH 8.0 Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O 100% activity Bacillus licheniformis L-aspartate + NH3
-
 ?
L-asparagine + H2O 100% activity Bacillus licheniformis MTCC 429 L-aspartate + NH3
-
 ?
L-aspartic acid + NH3 less than 3% activity compared to L-asparagine Bacillus licheniformis ?
-
 ?
L-aspartic acid + NH3 less than 3% activity compared to L-asparagine Bacillus licheniformis MTCC 429 ?
-
 ?
L-glutamine + H2O less than 1% activity compared to L-asparagine Bacillus licheniformis L-glutamate + NH3
-
 ?
L-glutamine + H2O less than 1% activity compared to L-asparagine Bacillus licheniformis MTCC 429 L-glutamate + NH3
-
 ?
additional information no activity with D-asparagine monohydrate and L-glutamic acid Bacillus licheniformis ?
-
 ?
additional information no activity with D-asparagine monohydrate and L-glutamic acid Bacillus licheniformis MTCC 429 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Bacillus licheniformis

Synonyms

Synonyms Comment Organism
ansA3
-
 Bacillus licheniformis
L-asparaginase III isoform Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
36.8
-
 L-asparagine at 37°C and pH 8.0 Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 50% activity between pH 7.0 and 9.0, the enzyme is inactive at pH 6.0 and 10.0 Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
54
-
 L-asparagine at 37°C and pH 8.0 Bacillus licheniformis