Cloned (Comment) | Organism |
---|---|
gene ansB, expression in Escherichia coli strain BL21 (DE3) | Yersinia pseudotuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
L-asparagine | pH 8.0, 37°C | Yersinia pseudotuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | the catalytic activity of YpA does not vary significantly as a function of ionic strength at 100-3000 mM KCl | Yersinia pseudotuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Yersinia pseudotuberculosis | - |
L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Yersinia pseudotuberculosis IP32953 | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pseudotuberculosis | Q66CJ2 | gene ansB | - |
Yersinia pseudotuberculosis IP32953 | Q66CJ2 | gene ansB | - |
Purification (Comment) | Organism |
---|---|
recombinant YpA from Escherichia coli strain BL21 (DE3) by two different steps of anion exchange chromatography | Yersinia pseudotuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.7 | - |
pH 8.0, 37°C, purified enzyme | Yersinia pseudotuberculosis |
Storage Stability | Organism |
---|---|
-70°C to -20°C, purified enzyme, pH 5.0-9.0, several weeks, no loss of activity | Yersinia pseudotuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Yersinia pseudotuberculosis | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Yersinia pseudotuberculosis IP32953 | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | YpA L-glutaminase activity is relatively low and more than 15 times less than specific activity towards L-Asn | Yersinia pseudotuberculosis | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | YpA L-glutaminase activity is relatively low and more than 15 times less than specific activity towards L-Asn | Yersinia pseudotuberculosis IP32953 | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase | - |
Yersinia pseudotuberculosis |
Ypa | - |
Yersinia pseudotuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Yersinia pseudotuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | 73 | over 80% of maximal activity within this range | Yersinia pseudotuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified enzyme, pH 5.0-9.0, 15% activity remaining after 3 min, 6% after 5 min | Yersinia pseudotuberculosis |
80 | - |
purified enzyme, pH 5.0-9.0, below 1% activity remaining after 10 min | Yersinia pseudotuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
L-asparagine | pH 8.0, 37°C | Yersinia pseudotuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Yersinia pseudotuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | over 80% of maximal activity within this range | Yersinia pseudotuberculosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Yersinia pseudotuberculosis | isoelectric focusing | - |
5.4 |
General Information | Comment | Organism |
---|---|---|
physiological function | antiproliferative effect of YpA in vitro and in vivo, overview | Yersinia pseudotuberculosis |