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Literature summary for 3.5.1.1 extracted from

  • Pokrovskaya, M.V.; Aleksandrova, S.S.; Pokrovsky, V.S.; Omeljanjuk, N.M.; Borisova, A.A.; Anisimova, N.Y.; Sokolov, N.N.
    Cloning, expression and characterization of the recombinant Yersinia pseudotuberculosis L-asparaginase (2012), Protein Expr. Purif., 82, 150-154.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ansB, expression in Escherichia coli strain BL21 (DE3) Yersinia pseudotuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
L-asparagine pH 8.0, 37°C Yersinia pseudotuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
KCl the catalytic activity of YpA does not vary significantly as a function of ionic strength at 100-3000 mM KCl Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Yersinia pseudotuberculosis
-
L-aspartate + NH3
-
?
L-asparagine + H2O Yersinia pseudotuberculosis IP32953
-
L-aspartate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Yersinia pseudotuberculosis Q66CJ2 gene ansB
-
Yersinia pseudotuberculosis IP32953 Q66CJ2 gene ansB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant YpA from Escherichia coli strain BL21 (DE3) by two different steps of anion exchange chromatography Yersinia pseudotuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.7
-
pH 8.0, 37°C, purified enzyme Yersinia pseudotuberculosis

Storage Stability

Storage Stability Organism
-70°C to -20°C, purified enzyme, pH 5.0-9.0, several weeks, no loss of activity Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
Yersinia pseudotuberculosis L-aspartate + NH3
-
?
L-asparagine + H2O
-
Yersinia pseudotuberculosis IP32953 L-aspartate + NH3
-
?
L-glutamine + H2O YpA L-glutaminase activity is relatively low and more than 15 times less than specific activity towards L-Asn Yersinia pseudotuberculosis L-glutamate + NH3
-
?
L-glutamine + H2O YpA L-glutaminase activity is relatively low and more than 15 times less than specific activity towards L-Asn Yersinia pseudotuberculosis IP32953 L-glutamate + NH3
-
?

Synonyms

Synonyms Comment Organism
L-asparaginase
-
Yersinia pseudotuberculosis
Ypa
-
Yersinia pseudotuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Yersinia pseudotuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
57 73 over 80% of maximal activity within this range Yersinia pseudotuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified enzyme, pH 5.0-9.0, 15% activity remaining after 3 min, 6% after 5 min Yersinia pseudotuberculosis
80
-
purified enzyme, pH 5.0-9.0, below 1% activity remaining after 10 min Yersinia pseudotuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
L-asparagine pH 8.0, 37°C Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Yersinia pseudotuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 over 80% of maximal activity within this range Yersinia pseudotuberculosis

pI Value

Organism Comment pI Value Maximum pI Value
Yersinia pseudotuberculosis isoelectric focusing
-
5.4

General Information

General Information Comment Organism
physiological function antiproliferative effect of YpA in vitro and in vivo, overview Yersinia pseudotuberculosis