Cloned (Comment) | Organism |
---|---|
gene ansA, phylogenetic analysis, expression of His-tagged AnsA in Escherichia coli strain BL21(DE3) | Rhizobium etli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aspartic acid | competitive | Rhizobium etli | |
beta-aspartyl hydroxamate | 48% inhibition at 5 mM | Rhizobium etli | |
Ca2+ | 31% inhibition at 1 mM | Rhizobium etli | |
D-asparagine | 39% inhibition at 5 mM | Rhizobium etli | |
Mg2+ | 31% inhibition at 1 mM | Rhizobium etli | |
Mn2+ | 52% inhibition at 1 mM | Rhizobium etli | |
additional information | poor inhibition by L-glutamine at 5 mM | Rhizobium etli | |
NH4+ | noncompetitive | Rhizobium etli | |
Zn2+ | 38% inhibition at 1 mM | Rhizobium etli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.9 | - |
L-asparagine | pH 7.5, 37°C, recombinant enzyme | Rhizobium etli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, recombinant His-tagged enzyme, SDS-PAGE | Rhizobium etli |
102000 | - |
recombinant His-tagged enzyme, native PAGE | Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | Q9RFN5 | gene ansA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AnsA 18fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Rhizobium etli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
purified recombinant enzyme, pH 7.5, 37°C | Rhizobium etli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Rhizobium etli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47000, recombinant His-tagged enzyme, SDS-PAGE | Rhizobium etli |
Synonyms | Comment | Organism |
---|---|---|
AnsA | - |
Rhizobium etli |
L-asparaginase | - |
Rhizobium etli |
L-asparaginase II | - |
Rhizobium etli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhizobium etli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | activity range, temperature profile overview | Rhizobium etli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinnat enzyme, loss of 60% activity after 20 min, inactivation within 30 min | Rhizobium etli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
106 | - |
L-asparagine | pH 7.5, 37°C, recombinant enzyme | Rhizobium etli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rhizobium etli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | activity range, pH profile overview | Rhizobium etli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
NH4+ | pH 7.5, 37°C, recombinant enzyme | Rhizobium etli | |
33 | - |
aspartic acid | pH 7.5, 37°C, recombinant enzyme | Rhizobium etli |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of an increasing rhizobial-type family of L-asparaginases | Rhizobium etli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
L-asparagine | pH 7.5, 37°C, recombinant enzyme | Rhizobium etli |