Application | Comment | Organism |
---|---|---|
medicine | L-asparaginase II from Erwinia carotovora may represent an important alternative therapy in the treatment of acute childhood lymphoblastic leukaemia, despite its promising lower glutaminase activity than Escherichia coli and Erwinia chrysanthemi L-asparaginases II | Pectobacterium carotovorum |
Cloned (Comment) | Organism |
---|---|
recombinant expression of L-asparaginase II with signal peptide, AspSP, in Escherichia coli strain BL21(DE3) in the periplasmic space and extracellular, and of L-asparaginase II without signal peptide, AspMP, in Escherichia coli strain C41(DE3) in the cytoplasm | Pectobacterium carotovorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Pectobacterium carotovorum | |
0.16 | - |
L-asparagine | pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide | Pectobacterium carotovorum | |
1.1 | - |
L-asparagine | pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide | Pectobacterium carotovorum | |
2.6 | - |
L-glutamine | pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide | Pectobacterium carotovorum | |
4.4 | - |
L-glutamine | pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide | Pectobacterium carotovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34500 | - |
x * 34500, about, sequence calculation, x * 39000, SDS-PAGE | Pectobacterium carotovorum |
39000 | - |
x * 34500, about, sequence calculation, x * 39000, SDS-PAGE | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Pectobacterium carotovorum | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant L-asparaginase II with signal peptide, AspSP, by cation exchange chromatography and gel filtration, and recombinant L-asparaginase II without signal peptide, AspMP, by a single-step cation exchange chromatography, to homogeneity | Pectobacterium carotovorum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
208.1 | - |
purified AspMP, enzyme without signal peptide, pH 8.0, 37°C | Pectobacterium carotovorum |
237.6 | - |
purified AspSP, enzyme with signal peptide, pH 8.0, 37°C | Pectobacterium carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | - |
Pectobacterium carotovorum | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34500, about, sequence calculation, x * 39000, SDS-PAGE | Pectobacterium carotovorum |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase II | - |
Pectobacterium carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pectobacterium carotovorum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
L-glutamine | pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide | Pectobacterium carotovorum | |
7.1 | - |
L-glutamine | pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide | Pectobacterium carotovorum | |
160 | - |
L-asparagine | pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide | Pectobacterium carotovorum | |
160 | - |
L-asparagine | pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide | Pectobacterium carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pectobacterium carotovorum |