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Literature summary for 3.5.1.1 extracted from

  • Saleem Basha, N.; Rekha, R.; Komala, M.; Ruby, S.
    Production of extracellular anti-leukaemic enzyme L-asparaginase from marine actinomycetes by solid-state and submerged fermentation: Purification and characterisation (2009), Trop. J. Pharm. Res., 8, 353-360.
No PubMed abstract available
Search for more literature for 3.5.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 L-asparagine pH 8.6, 37°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ inhibits enzyme, 1, 3, and 10 mM tested Streptomyces sp.
EDTA inhibits enzyme, 1, 3, and 10 mM tested Streptomyces sp.
Mn2+ activates enzyme, 1, 3, and 10 mM tested Streptomyces sp.
Zn2+ inhibits enzyme, 1, 3, and 10 mM tested Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 marine actinomycetes, soil isolate S1, S2, S3, S4, S5, S6, S8. K2, K4, K5, and K8, whereof S3, S4 and S6 are designated as Streptomyces spp.
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by ammonium sulfate precipitation, dialysis, gel filtration on Sephadex G-100 and SDS-PAGE Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
soil sediment samples obtained from Tamilnadu and Kerala in India, production of the enzyme in three different media, solid-State media, tryptone glucose yeast extract, and tryptone fructose yeast extract broth. 10 isolates (S1, S2, S3, S4, S5, S6, S8. K2, K4, K5, and K8), only S3, S4 and K8 show L-asparaginase activity Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
662.6
-
 purified enzyme, 37°C, pH 8.6 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Streptomyces sp. L-aspartate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
L-asparaginase
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces sp.
50
-
-
 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 ca. 30 U/ml at 30°C and 75°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 maximum activity between pH 7.0 and 8.0 with 80% activity at physiological pH Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 20 U/ml enzyme activity at pH 5 and 10 Streptomyces sp.
8.6
-
 assay at Streptomyces sp.