Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
- |
Streptomyces gulbargensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces gulbargensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
82.12fold using ammonium sulfate precipitation, gel filtration on Sephacryl and CM Sephadex C-50 gel filtration, 32% yield | Streptomyces gulbargensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2053 | - |
pH 8.5, 40°C | Streptomyces gulbargensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Streptomyces gulbargensis | L-aspartate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase | - |
Streptomyces gulbargensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces gulbargensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
the enzyme retains 55% of the activity at 80°C for 60 min in alkaline pH. Overview of effect of various pH and temperature on L-asparaginase production | Streptomyces gulbargensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces gulbargensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 80% of the activity in the pH-range of 7-10 | Streptomyces gulbargensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is more stable at the alkaline pH than at the acidic one and it retains 55% of the activity at 80°C for 60 min. 80% of the activity in the pH-range of 7-10. Overview of effect of various pH and temperature on L-asparaginase production | Streptomyces gulbargensis |