Application | Comment | Organism |
---|---|---|
medicine | chemotherapeutic agent | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
L-glutamine | kcat/Km: 0.48 1/mM*s, pH7.5, 37°C | Helicobacter pylori | |
additional information | - |
L-asparagine | kcat/Km: 66.41 1/mM*s, pH7.5, 37°C | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
subunit molecular weight, determined by SDS-PAGE | Helicobacter pylori |
140000 | - |
gel filtration | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | B6ZCD8 | CCUG 17874 | - |
Helicobacter pylori CCUG 17874 | B6ZCD8 | CCUG 17874 | - |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Helicobacter pylori | L-aspartic acid + NH3 | - |
? | |
L-asparagine + H2O | - |
Helicobacter pylori CCUG 17874 | L-aspartic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Helicobacter pylori | L-glutamic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Helicobacter pylori CCUG 17874 | L-glutamic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 37000, SDS-PAGE | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase | type II | Helicobacter pylori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
absolutely stable up to 50°C (10 min incubation time), 50% activity after 10 min at 53°C | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.26 | - |
L-asparagine | - |
Helicobacter pylori | |
22.1 | - |
L-glutamine | - |
Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | broad optimum for asparaginase activity | Helicobacter pylori |
7.5 | - |
glutaminase activity | Helicobacter pylori |