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Literature summary for 3.5.1.1 extracted from
- Extracellular expression and single step purification of recombinant Escherichia coli L-asparaginase II (2004), Protein Expr. Purif., 38, 29-36.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the N-terminally His-tagged enzyme fused to the pelB leader sequence under control of the T7lac promoter in strain BLR(DE3), the recombinant enzyme is secreted to the cell culture medium | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
4 * 37000, recombinant enzyme, SDS-PAGE | Escherichia coli |
| 150000 | - |
recombinant enzyme, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | Escherichia coli | - |
L-aspartate + NH3 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
isozyme II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 3.3fold from strain BLR(DE3) culture supernatant by nickel affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 190 | - |
purified recombinant enzyme | Escherichia coli |
| 200 | - |
purified native enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 37000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-asparaginase II | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Escherichia coli |
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