Literature summary for 3.5.1.1 extracted from

Michalska, K.; Bujacz, G.; Jaskolski, M.
Crystal structure of plant asparaginase (2006), J. Mol. Biol., 360, 105-116.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme performs autocatalytic activation	Lupinus luteus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged enzyme	Lupinus luteus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, protein solution at 10 mg/ml is mixed in 2:1 ratio with a solution containing 25% w/v PEG 4000, 100 mM HEPES, pH 6.5, and 200 mM MgCl2, incubation overnight at room temperature, the precipitate is centrifuged and the supernatant is diluted 1:1 with water, was used for hanging-drop vapor diffusion, 0.003 ml solution is mixed with 0.001 ml of reservoir solution containing 20% PEG 4000, 100 mM HEPES, pH 6.5, and 200 mM MgCl2, 200 mM MgCl2, streak-seeding with nuclei, room temperature, one day, X-ray diffraction structure determination and analysis at 2.6 A resolution, structure modelling	Lupinus luteus

Crystallization (Comment)

Organism

purified recombinant enzyme, protein solution at 10 mg/ml is mixed in 2:1 ratio with a solution containing 25% w/v PEG 4000, 100 mM HEPES, pH 6.5, and 200 mM MgCl2, incubation overnight at room temperature, the precipitate is centrifuged and the supernatant is diluted 1:1 with water, was used for hanging-drop vapor diffusion, 0.003 ml solution is mixed with 0.001 ml of reservoir solution containing 20% PEG 4000, 100 mM HEPES, pH 6.5, and 200 mM MgCl2, 200 mM MgCl2, streak-seeding with nuclei, room temperature, one day, X-ray diffraction structure determination and analysis at 2.6 A resolution, structure modelling

Lupinus luteus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Na+	probably, metal binding loop structure involving Leu59, Glu60, Ile62, Phe65, Ala67, and Ile69, overview	Lupinus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-asparagine + H2O	Lupinus luteus	-	L-aspartate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Lupinus luteus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme, removal of His-tag by thrombin, anion exchange chromatography and gel filtration	Lupinus luteus

Reaction

Reaction	Comment	Organism	Reaction ID
L-asparagine + H2O = L-aspartate + NH3	active site structure involving Thr193, autocatalytic activation mechanism involving Thr193	Lupinus luteus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-asparagine + H2O	-	Lupinus luteus	L-aspartate + NH3	-	?
additional information	the enzyme performs autocatalytic activation, overview	Lupinus luteus	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	(alphabeta)2, a dimer of heterodimers, quarternary structure, crystal structure, overview	Lupinus luteus

Synonyms

Synonyms	Comment	Organism
L-asparaginase	-	Lupinus luteus