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Literature summary for 3.5.1.1 extracted from

  • Gaofu, Q.; Jie, L.; Rongyue, C.; Xin, Y.; Dan, M.; Jie, W.; Xiangchun, S.; Qunwei, X.; Roque, R.S.; Xiuyun, Z.; Jingjing, L.
    Asparaginase display of polypeptides in the periplasm of Escherichia coli: potential rapid pepscan technique for antigen epitope mapping (2005), J. Immunol. Methods, 299, 9-19.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the mutant chimeric enzyme in the periplasm of strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric enzyme composed of asparaginase, a tetanus toxin peptide spacer, fragment 831-854, and the foreign cholesteryl ester transfer protein C-terminal fragment, targeting to and expression in the periplasm of Escherichia coli Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Escherichia coli
-
L-aspartate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native wild-type and recombinant mutant chimeric enzyme from the periplasm of strain BL21(DE3) by periplasm preparation through osmotic shock, and anion exchange chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
Escherichia coli L-aspartate + NH3
-
?

Subunits

Subunits Comment Organism
More potential rapid pepscan technique for antigen epitope mapping Escherichia coli
tetramer homotetramer Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli