Application | Comment | Organism |
---|---|---|
medicine | the enzyme can be efficiently immobilized on epoxy-activated Sepharose CL-6B. The immobilized enzyme retains most of its activity (60%) and shows high stability at 4°C. The approach offers the possibility of designing an L-asparaginase from Erwinia chrysanthemi bioreactor that can be operated over a long period of time with high efficiency, which can be used in leukaemia therapy | Dickeya chrysanthemi |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3)pLysS | Dickeya chrysanthemi |
expression in Escherichia coli strain BL21(DE3) | Dickeya chrysanthemi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the purified recombinant enzyme on epoxy-activated resin, the immobilized enzyme retains 60% of maximal activity and is highly stable at 4°C, utilization as bioreactor | Dickeya chrysanthemi |
General Stability | Organism |
---|---|
the enzyme can be efficiently immobilized on epoxy-activated Sepharose CL-6B. The immobilized enzyme retains most of its activity (60%) and shows high stability at 4°C | Dickeya chrysanthemi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Dickeya chrysanthemi | |
0.058 | - |
L-asparagine | pH 8, 37°C | Dickeya chrysanthemi | |
0.8 | - |
Nalpha-acetyl-Asn | pH 8, 37°C | Dickeya chrysanthemi | |
6.7 | - |
L-Gln | pH 8.2, 37°C | Dickeya chrysanthemi | |
18.8 | - |
succinamic acid | pH 8, 37°C | Dickeya chrysanthemi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Dickeya chrysanthemi | - |
L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Dickeya chrysanthemi 3937 | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | - |
- |
- |
Dickeya chrysanthemi | Q6Q4F3 | - |
- |
Dickeya chrysanthemi 3937 | - |
- |
- |
Dickeya chrysanthemi 3937 | Q6Q4F3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Dickeya chrysanthemi |
recombinant enzyme from Escherichia coli strain BL21(DE3) in a single step procedure to homogeneity by cation exchange | Dickeya chrysanthemi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Dickeya chrysanthemi | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Dickeya chrysanthemi 3937 | L-aspartate + NH3 | - |
? | |
L-Gln + H2O | - |
Dickeya chrysanthemi | L-Glu + NH3 | - |
? | |
L-Gln + H2O | - |
Dickeya chrysanthemi 3937 | L-Glu + NH3 | - |
? | |
Nalpha-acetyl-L-Asn + H2O | - |
Dickeya chrysanthemi | Nalpha-acetyl-L-Asp + NH3 | - |
? | |
Nalpha-acetyl-L-Asn + H2O | - |
Dickeya chrysanthemi 3937 | Nalpha-acetyl-L-Asp + NH3 | - |
? | |
succinamic acid + H2O | - |
Dickeya chrysanthemi | succinate + NH3 | - |
? | |
succinamic acid + H2O | - |
Dickeya chrysanthemi 3937 | succinate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASNase | - |
Dickeya chrysanthemi |
L-ASNase | - |
Dickeya chrysanthemi |
L-asparaginase | - |
Dickeya chrysanthemi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
particularly stable at | Dickeya chrysanthemi |
46 | - |
enzyme shows higher thermal stability at acidic to neutral pH values, lower stability is observed at alkaline pH. The inactivation profiles at pH 5.5 and 7 are biphasic and shows two clear transitions with inflection points corresponding to Tm values of 46.6°C and 62.5°C at pH 5.5, and 46.4 and 57.2°C at pH 7, respectively | Dickeya chrysanthemi |
47 | - |
enzyme shows higher thermal stability at acidic to neutral pH values, lower stability is observed at alkaline pH. The inactivation profiles at pH 5.5 and 7 are biphasic and shows two clear transitions with inflection points corresponding to Tm values of 46.6°C and 62.5°C at pH 5.5, and 46.4 and 57.2°C at pH 7, respectively | Dickeya chrysanthemi |
57 | - |
enzyme shows higher thermal stability at acidic to neutral pH values, lower stability is observed at alkaline pH. The inactivation profiles at pH 5.5 and 7 are biphasic and shows two clear transitions with inflection points corresponding to Tm values of 46.6°C and 62.5°C at pH 5.5, and 46.4 and 57.2°C at pH 7, respectively | Dickeya chrysanthemi |
63 | - |
enzyme shows higher thermal stability at acidic to neutral pH values, lower stability is observed at alkaline pH. The inactivation profiles at pH 5.5 and 7 are biphasic and shows two clear transitions with inflection points corresponding to Tm values of 46.6°C and 62.5°C at pH 5.5, and 46.4 and 57.2°C at pH 7, respectively | Dickeya chrysanthemi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
L-Gln | pH 8.2, 37°C | Dickeya chrysanthemi | |
0.0108 | - |
Nalpha-acetyl-Asn | pH 8, 37°C | Dickeya chrysanthemi | |
0.0128 | - |
L-asparagine | pH 8, 37°C | Dickeya chrysanthemi | |
0.0239 | - |
succinamic acid | pH 8, 37°C | Dickeya chrysanthemi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dickeya chrysanthemi | pI calculated from sequence is 8.57 | - |
8.6 |