Literature summary for 3.5.1.1 extracted from

Yao, M.; Yasutake, Y.; Morita, H.; Tanaka, I.
Structure of the type I L-asparaginase from the hyperthermophilic archaeon Pyrococcus horikoshii at 2.16 A resolution (2005), Acta Crystallogr. Sect. D, D61, 294-301.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene PH0066, expression in Escherichia coli strain BL21	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, 0.002 ml or protein solution is mixed with 0.001 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 12% v/v PEG 400, and 30 mM NaCl, soaking of crystals in 25% v/v PEG 400 and flash-cooling, X-ray difraction structure determination and analysis at 2.16 A resolution	Pyrococcus horikoshii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Pyrococcus horikoshii	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-asparagine + H2O	Pyrococcus horikoshii	-	L-aspartate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PhA from Escherichia coli strain BL21 by anion exchange chromatography and gel filtration, followed by hydrophobic interaction chromatography and ultrafiltration	Pyrococcus horikoshii

Reaction

Reaction	Comment	Organism	Reaction ID
L-asparagine + H2O = L-aspartate + NH3	active site structure with a flexible loop that is stabilized by a beta-hairpin formation and intracellular interaction with the alpha-helical region, modelling	Pyrococcus horikoshii	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-asparagine + H2O	-	Pyrococcus horikoshii	L-aspartate + NH3	-	?

Subunits

Subunits	Comment	Organism
dimer	homodimer, crystal structure	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
L-asparagine aminohydrolase	-	Pyrococcus horikoshii
PhA	-	Pyrococcus horikoshii
type I L-asparaginase	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)