Application | Comment | Organism |
---|---|---|
medicine | potent antineoplastic agent in animals which has given complete remission in some human leukemias | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asn + H2O | Escherichia coli | - |
L-Asp + NH3 | - |
? | |
L-Asn + H2O | Escherichia coli HAP | - |
L-Asp + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
highest yield in aerobically grown cells in corn steep medium, addition of L-glutamic acid, L-methionine, and lactic acid enhances enzyme production, addition of glucose or sodium depresses enzyme production | - |
Escherichia coli HAP | - |
highest yield in aerobically grown cells in corn steep medium, addition of L-glutamic acid, L-methionine, and lactic acid enhances enzyme production, addition of glucose or sodium depresses enzyme production | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
620 | - |
pH 8.0, 37°C | Escherichia coli |
Storage Stability | Organism |
---|---|
5°C lyphilyzed enzyme, stable upon storage for at least several weeks | Escherichia coli |
5°C, sterile enzyme solution with high specific activity, several weeks, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asn + H2O | - |
Escherichia coli | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Escherichia coli HAP | L-Asp + NH3 | - |
? |