Literature summary for 3.5.1.1 extracted from

Krasotkina, J.; Borisova, A.A.; Gervaziev, Y.V.; Sokolov, N.N.
One-step purification and kinetic properties of the recombinant L-asparaginase from Erwinia carotovora (2004), Biotechnol. Appl. Biochem., 39, 215-221.

Search for more literature for 3.5.1.1

Application

Application	Comment	Organism
medicine	a prospective therapeutic enzyme for leukemia treatment	Pectobacterium carotovorum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pectobacterium carotovorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00016	-	L-Asn	37°C	Pectobacterium carotovorum
0.442	-	L-aspartyl-beta-hydroxamate	37°C	Pectobacterium carotovorum
0.832	-	D-Asn	37°C	Pectobacterium carotovorum
3.44	-	L-Gln	37°C	Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	x * 34000, SDS-PAGE	Pectobacterium carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
630	-	-	Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Asn + H2O	-	Pectobacterium carotovorum	D-Asp + NH3	-	?
L-Asn + H2O	-	Pectobacterium carotovorum	L-Asp + NH3	-	?
L-aspartyl-beta-hydroxamate + H2O	-	Pectobacterium carotovorum	L-Asp + hydroxylamine	-	?
L-Gln + H2O	-	Pectobacterium carotovorum	L-Glu + NH3	-	?

Subunits

Subunits	Comment	Organism
?	x * 34000, SDS-PAGE	Pectobacterium carotovorum

Synonyms

Synonyms	Comment	Organism
ECAR-LANS	-	Pectobacterium carotovorum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
42	-	L-Gln	37°C	Pectobacterium carotovorum
68	-	D-Asn	37°C	Pectobacterium carotovorum
450	-	L-aspartyl-beta-hydroxamate	37°C	Pectobacterium carotovorum
1600	-	L-Asn	37°C	Pectobacterium carotovorum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	9	pH 6.5: about 70% of the ativity at pH 9.0, pH 9.0: activity increases from pH 5.5 to pH 9.0	Pectobacterium carotovorum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pectobacterium carotovorum	isoelectric-focusing, pH-gradient 3.5-9.5	-	8.5

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)