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Literature summary for 3.5.1.1 extracted from
- Purification, characterization and antitumor activity of L-asparaginase isolated from Pseudomonas stutzeri MB-405 (1995), Curr. Microbiol., 30, 291-298.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | enhances activity | Pseudomonas stutzeri |  |
| dithiothreitol | enhances activity | Pseudomonas stutzeri | |
| EDTA | enhances activity | Pseudomonas stutzeri | |
| glutathione | enhances activity | Pseudomonas stutzeri | |
| GSH | enhances activity | Pseudomonas stutzeri | |
| L-Cys | enhances activity | Pseudomonas stutzeri | |
| L-His | enhances activity | Pseudomonas stutzeri | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme has antitumor activity | Pseudomonas stutzeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Diazo-4-oxo-L-norvaline | - |
Pseudomonas stutzeri | |
| Ca2+ | - |
Pseudomonas stutzeri | |
| Cd2+ | - |
Pseudomonas stutzeri | |
| Cu2+ | - |
Pseudomonas stutzeri | |
| Fe3+ | - |
Pseudomonas stutzeri | |
| Hg2+ | - |
Pseudomonas stutzeri | |
| iodoacetamide | - |
Pseudomonas stutzeri | |
| Mg2+ | - |
Pseudomonas stutzeri | |
| Mn2+ | - |
Pseudomonas stutzeri | |
| Ni2+ | - |
Pseudomonas stutzeri | |
| PCMB | - |
Pseudomonas stutzeri | |
| SDS | - |
Pseudomonas stutzeri | |
| Urea | - |
Pseudomonas stutzeri | |
| Zn2+ | - |
Pseudomonas stutzeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.145 | - |
L-Asn | - |
Pseudomonas stutzeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates | Pseudomonas stutzeri | |
| Mg2+ | stimulates | Pseudomonas stutzeri | |
| Na+ | activates | Pseudomonas stutzeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000, SDS-PAGE | Pseudomonas stutzeri |
| 34000 | - |
gel filtration | Pseudomonas stutzeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
MB-405 | - |
| Pseudomonas stutzeri MB-405 | - |
MB-405 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 732.3 | - |
- |
Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-cyano-L-Ala + H2O | 9.5% of the activity with L-Asn | Pseudomonas stutzeri | ? | - |
? | |
| beta-cyano-L-Ala + H2O | 9.5% of the activity with L-Asn | Pseudomonas stutzeri MB-405 | ? | - |
? | |
| L-Asn + H2O | - |
Pseudomonas stutzeri | L-Asp + NH3 | - |
? | |
| L-Asn + H2O | - |
Pseudomonas stutzeri MB-405 | L-Asp + NH3 | - |
? | |
| L-aspartic acid beta-hydroxamate + H2O | 11% of the activity with L-Asn | Pseudomonas stutzeri | L-Asp + hydroxylamine | - |
? | |
| L-aspartic acid beta-hydroxamate + H2O | 11% of the activity with L-Asn | Pseudomonas stutzeri MB-405 | L-Asp + hydroxylamine | - |
? | |
| L-Gln + H2O | - |
Pseudomonas stutzeri | L-Glu + NH3 | - |
? | |
| L-Gln + H2O | - |
Pseudomonas stutzeri MB-405 | L-Glu + NH3 | - |
? | |
| additional information | enzyme has antitumor activity | Pseudomonas stutzeri | ? | - |
? | |
| additional information | enzyme has antitumor activity | Pseudomonas stutzeri MB-405 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, SDS-PAGE | Pseudomonas stutzeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas stutzeri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | 30°C: about 60% of maximal activity, 55°C: about 55% of maximal activity | Pseudomonas stutzeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
60 min, no significant loss of activity | Pseudomonas stutzeri |
| 60 | - |
half-life: 25 min | Pseudomonas stutzeri |
| 65 | - |
half-life: 10 min | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas stutzeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.5 | pH 6.5: about 45% of maximal activity, pH 10.5: about 65% of maximal activity | Pseudomonas stutzeri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | maximal stability in the range | Pseudomonas stutzeri |
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