Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Acinetobacter calcoaceticus | |
Cu2+ | - |
Acinetobacter calcoaceticus | |
Hg2+ | - |
Acinetobacter calcoaceticus | |
NH4+ | - |
Acinetobacter calcoaceticus | |
Ni2+ | - |
Acinetobacter calcoaceticus | |
PCMB | reversed by mercaptoethanol | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
L-asparagine | - |
Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Acinetobacter calcoaceticus |
105000 | - |
gel filtration | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Acinetobacter calcoaceticus |
Storage Stability | Organism |
---|---|
-20°C, little loss of activity after prolonged storage | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Asn + H2O | at 5% of the activity with L-Asn | Acinetobacter calcoaceticus | D-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Acinetobacter calcoaceticus | L-Asp + NH3 | - |
? | |
L-Gln + H2O | at 10% of the activity with L-Asn | Acinetobacter calcoaceticus | L-Glu + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25000, SDS-PAGE | Acinetobacter calcoaceticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
3 h, stable | Acinetobacter calcoaceticus |
60 | - |
5 min, complete inactivation | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.8 | - |
Acinetobacter calcoaceticus |