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Literature summary for 3.5.1.1 extracted from

  • Lea, P.J.; Fowden, L.; Miflin, B.J.
    The purification and properties of asparaginase from Lupinus species (1978), Phytochemistry, 17, 217-222.
No PubMed abstract available
Search for more literature for 3.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
PCMB slight stimulation Lupinus polyphyllus
Tartrate stimulation Lupinus polyphyllus

Inhibitors

Inhibitors Comment Organism Structure
2-amino-5-chloro-4-oxo pentanoic acid
-
 Lupinus polyphyllus
3-Cyano-L-Ala
-
 Lupinus polyphyllus
4-methylene-L-glutamine
-
 Lupinus polyphyllus
5-Bromo-4-oxo-L-norvaline
-
 Lupinus polyphyllus
Br-
-
 Lupinus polyphyllus
Cd2+
-
 Lupinus polyphyllus
Co2+
-
 Lupinus polyphyllus
Cu2+
-
 Lupinus polyphyllus
Dichromate
-
 Lupinus polyphyllus
DL-aspartate 3-hydroxamate
-
 Lupinus polyphyllus
Fe2+
-
 Lupinus polyphyllus
Mg2+
-
 Lupinus polyphyllus
N4-hydroxyethyl-L-asparagine
-
 Lupinus polyphyllus
Ni2+
-
 Lupinus polyphyllus
Pb4+
-
 Lupinus polyphyllus
Sn2+
-
 Lupinus polyphyllus
sulfate
-
 Lupinus polyphyllus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.2
-
 L-asparagine
-
 Lupinus polyphyllus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight stimulation Lupinus polyphyllus
diphosphate stimulation Lupinus polyphyllus
HPO42- stimulation Lupinus polyphyllus
K3Fe(CN)6 stimulation Lupinus polyphyllus
Na+ slight stimulation Lupinus polyphyllus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Lupinus polyphyllus
72000
-
 sucrose density gradient centrifugation Lupinus polyphyllus

Organism

Organism UniProt Comment Textmining
Lupinus polyphyllus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lupinus polyphyllus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Lupinus polyphyllus
-

Storage Stability

Storage Stability Organism
-20°C, less than 10% loss of activity after 3 months Lupinus polyphyllus
4°C, stable for up to 4 days Lupinus polyphyllus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-aspartyl hydroxamate + H2O
-
 Lupinus polyphyllus DL-Asp + hydroxylamine
-
 ?
L-Asn + H2O
-
 Lupinus polyphyllus L-Asp + NH3
-
 ?
L-Gln + H2O no activity Lupinus polyphyllus L-Glu + NH3
-
 ?
N4-ethyl-L-asparagine 12% of the activity with L-Asn Lupinus polyphyllus L-Asp + propylamine
-
 ?
N4-methoxy-L-asparagine + H2O 154% of the activity with L-Asn Lupinus polyphyllus L-Asp + O-methylhydroxylamine
-
 ?
N4-methyl-L-asparagine 12% of the activity with L-Asn Lupinus polyphyllus L-Asp + methylamine
-
 ?
threo-3-hydroxy-L-asparagine + H2O 36% of the activity with L-Asn Lupinus polyphyllus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Lupinus polyphyllus