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Literature summary for 3.4.99.B2 extracted from

  • Nirasawa, S.; Nakahara, K.; Takahashi, S.
    Cloning and characterization of the novel D-aspartyl endopeptidase, paenidase, from Paenibacillus sp. B38 (2018), J. Biochem., 164, 103-112 .
    View publication on PubMed
Search for more literature for 3.4.99.B2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenibacillus sp. B38

Protein Variants

Protein Variants Comment Organism
H276A about 35% of wild-type specific activity Paenibacillus sp. B38

Inhibitors

Inhibitors Comment Organism Structure
benzoyl-Arg-His-[D-Asp]-CH2Cl 0.1 mM, 48% residual activity Paenibacillus sp. B38
Mn2+ 0.1 mM, 68% residual activity Paenibacillus sp. B38
additional information not inhibitory: pepstatin, Co2+ Paenibacillus sp. B38
Zn2+ 0.1 mM, 46% residual activity Paenibacillus sp. B38

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 succinyl-D-Asp-7-amido-4-methylcoumarin mutant H276A, pH 7.0, 37°C Paenibacillus sp. B38
0.97
-
 succinyl-D-Asp-7-amido-4-methylcoumarin wild-type, pH 7.0, 37°C Paenibacillus sp. B38

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Paenibacillus sp. B38
-
-

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. B38 A0A2Z6BCG6
-
-

Purification (Commentary)

Purification (Comment) Organism
purification from cell filtrate Paenibacillus sp. B38

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
737
-
 mutant H276A, pH 8.0, 37°C Paenibacillus sp. B38
2225
-
 wild-type, pH 8.0, 37°C Paenibacillus sp. B38

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme specifically recognizes an internal D-Asp residue to cleave [D-Asp]-X peptide bonds Paenibacillus sp. B38 ?
-
 ?
succinyl-D-Asp-7-amido-4-methylcoumarin + H2O
-
 Paenibacillus sp. B38 succinyl-D-Asp + 7-amino-4-methylcoumarin
-
 ?
succinyl-D-Asp-p-nitroanilide + H2O
-
 Paenibacillus sp. B38 succinyl-D-Asp + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, mature recombinant protein, x * 55000, recombinant pro-protein, SDS-PAGE, x * 55756, pro-orotein, calculated from sequence Paenibacillus sp. B38

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below Paenibacillus sp. B38

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
 succinyl-D-Asp-7-amido-4-methylcoumarin mutant H276A, pH 7.0, 37°C Paenibacillus sp. B38
2.88
-
 succinyl-D-Asp-7-amido-4-methylcoumarin wild-type, pH 7.0, 37°C Paenibacillus sp. B38

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Paenibacillus sp. B38

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.37
-
 succinyl-D-Asp-7-amido-4-methylcoumarin mutant H276A, pH 7.0, 37°C Paenibacillus sp. B38
2.97
-
 succinyl-D-Asp-7-amido-4-methylcoumarin wild-type, pH 7.0, 37°C Paenibacillus sp. B38