Protein Variants | Comment | Organism |
---|---|---|
C251A | wild-type in function | Saccharomyces cerevisiae |
E156A | activity only when overexpressed | Saccharomyces cerevisiae |
E156D | marginal activity, overexpression significantly boosts the activity | Saccharomyces cerevisiae |
E156Q | no activity | Saccharomyces cerevisiae |
E157A | diminished but significant activity | Saccharomyces cerevisiae |
F190A | diminished but significant activity | Saccharomyces cerevisiae |
H194A | invariably inactive | Saccharomyces cerevisiae |
H196A | wild-type in function | Saccharomyces cerevisiae |
H197A | wild-type in function | Saccharomyces cerevisiae |
H248A | activity only when overexpressed | Saccharomyces cerevisiae |
additional information | strain yWS163, that lacks the a-factor encoding genes and the CAAX protease genes | Saccharomyces cerevisiae |
N252A | diminished but significant activity | Saccharomyces cerevisiae |
Y160A | diminished but significant activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00144 | - |
a-factor derived synthetic farnesylated pentadecapeptide | mutant H197A | Saccharomyces cerevisiae | |
0.00172 | - |
a-factor derived synthetic farnesylated pentadecapeptide | wild type | Saccharomyces cerevisiae | |
0.00234 | - |
a-factor derived synthetic farnesylated pentadecapeptide | mutant C251A | Saccharomyces cerevisiae | |
0.00326 | - |
a-factor derived synthetic farnesylated pentadecapeptide | mutant H196A | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
membrane | - |
Arabidopsis thaliana | 16020 | - |
membrane | - |
Caenorhabditis elegans | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Caenorhabditis elegans | - |
- |
- |
Drosophila melanogaster | - |
- |
- |
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
strain RC757, strain SM1068, strain SM3614, strain STY50 | - |
Schizosaccharomyces pombe | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a-factor derived synthetic farnesylated pentadecapeptide + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
CALM + H2O | - |
Homo sapiens | ? | - |
? | |
CALM + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
CALQ + H2O | - |
Homo sapiens | ? | - |
? | |
CALQ + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
CAMQ + H2O | - |
Homo sapiens | ? | - |
? | |
CAMQ + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
CASQ + H2O | no cleavage when RCE1 is expressed from low copy plasmids or is overexpressed | Arabidopsis thaliana | ? | - |
? | |
CASQ + H2O | no cleavage when RCE1 is expressed from low copy plasmids or is overexpressed | Caenorhabditis elegans | ? | - |
? | |
CASQ + H2O | no cleavage when RCE1 is expressed from low copy plasmids, but overexpressed RCE1 appears to have marginal activity towards CASQ | Saccharomyces cerevisiae | ? | - |
? | |
CSVM + H2O | - |
Homo sapiens | ? | - |
? | |
CSVM + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
CTLM + H2O | - |
Homo sapiens | ? | - |
? | |
CTLM + H2O | one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed | Saccharomyces cerevisiae | ? | - |
? | |
CTLM + H2O | one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed | Arabidopsis thaliana | ? | - |
? | |
CTLM + H2O | one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed | Caenorhabditis elegans | ? | - |
? | |
CTSM + H2O | - |
Homo sapiens | ? | - |
? | |
CTSM + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
CTVM + H2O | - |
Homo sapiens | ? | - |
? | |
CTVM + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
CVIA + H2O | - |
Homo sapiens | ? | - |
? | |
CVIA + H2O | one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed | Saccharomyces cerevisiae | ? | - |
? | |
CVIA + H2O | one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed | Arabidopsis thaliana | ? | - |
? | |
CVIA + H2O | one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed | Caenorhabditis elegans | ? | - |
? | |
additional information | CTSQ motif poorly cleaved | Homo sapiens | ? | - |
? | |
additional information | CTSQ motif poorly cleaved | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ras-converting enzyme | - |
Drosophila melanogaster |
Ras-converting enzyme | - |
Homo sapiens |
Ras-converting enzyme | - |
Saccharomyces cerevisiae |
Ras-converting enzyme | - |
Arabidopsis thaliana |
Ras-converting enzyme | - |
Schizosaccharomyces pombe |
Ras-converting enzyme | - |
Caenorhabditis elegans |
RCE1 | - |
Drosophila melanogaster |
RCE1 | - |
Homo sapiens |
RCE1 | - |
Saccharomyces cerevisiae |
RCE1 | - |
Arabidopsis thaliana |
RCE1 | - |
Schizosaccharomyces pombe |
RCE1 | - |
Caenorhabditis elegans |
Rce1p | - |
Saccharomyces cerevisiae |